侵染天南星科植物病毒的分子鉴定及其生态学研究

应用生态学报 ›› 2004, Vol. ›› Issue (4): 566-570.

侵染天南星科植物病毒的分子鉴定及其生态学研究

刘文洪, 陈集双, 李永伟

浙江大学生命科学学院, 杭州 310029

收稿日期:2002-10-12 修回日期:2003-02-28 出版日期:2004-04-15
通讯作者: 陈集双
基金资助:
国家自然科学基金资助项目(3988028)

Molecular identification of Araceae-infecting viruses and their ecology

LIU Wenhong, CHEN Jishuang, LI Yongwei

College of Life Science, Zhejiang University, Hangzhou 310029, China

Received:2002-10-12 Revised:2003-02-28 Online:2004-04-15

摘要/Abstract

摘要： 通过病毒粒子部分提纯和形态学观察,发现侵染我国南方天南星科植物的病毒主要有线状和球状两种形态.经病毒基因组序列分析确定线状病毒为芋花叶病毒(DsMV);经血清学反应和序列分析确定球状病毒为黄瓜花叶病毒(CMV).CMVCP基因序列同源性分析的结果表明,侵染天南星科的CMV是相对独立的种内变异类型,归属于亚组I.同时,CMV存在对天南星科植物的适应性变异.对采自我国海南、湖南、浙江、上海等地的126个天南星科植物样品进行RNA核酸斑点杂交检测,获得病毒检测结果.海南省样品DsMV的检出率为73.3%,CMV的检出率为46.7%;湖南省样品DsMV的检出率为100%,CMV的检出率为38.5%;浙江省样品DsMV的检出率为93.0%,CMV的检出率为7.0%;上海市样品DsMV的检出率为100%,尚没有检测到CMV.首次证实了自然条件下CMV作为天南星科植物主要病毒的存在,在我国南方地区,该病毒对天南星科植物的自然侵染受到气候、季节和寄主等生态因子的影响.DsMV则在天南星科植物上普遍存在.

关键词: 天南星科, 黄瓜花叶病毒, 芋花叶病毒, 核酸斑点杂交, 序列分析

Abstract: A filamentous virus and a spherical virus were detected from partial purification with natural-infected Araceae plants.The filamentous virus was confirmed as dasheen mosaic virus (DsMV) by RNA spot hybridization (RSH) and sequence determination,while the spherical virus was identified as a new strain of cucumber mosaic virus (CMV) by enzyme-linked immunosorbent assay and sequence determination of the 3' end genes of RNA3.CMV was firstly confirmed as one of the principal viruses infecting the Araceae plants under natural condition.According to the sequence similarity of coat protein,Araceae-infecting CMV was then decided belonging to CMV sub-group I,but independent from other isolates in this subgroup.By dot-RNA hybridization to DsMV and CMV probes with total RNA extracted from leaf tissue,field samples collected from some parts of southern China were detected for their natural infection.The infection ratio of field samples infected by DsMV and CMV were 73.3% and 46.7% for samples collected from Hainan Province,100% and 38.5% for those from Hunan Province,93.0% and 38.5% for that from Zhejiang Province,and 100% and no infection for samples from Shanghai,respectively.The infection of viruses on Araceae plants was obviously affected by such ecological factors as geological distribution,climate and host species.The occurrence of CMV was greatly influenced by the above factors,but DsMV always existed as the most commonly distributed virus.

Key words: Araceae, Cucumber mosaic virus, Dasheen mosaic virus, Nucleic acid spot hybridization, Sequence determination

中图分类号:

Q939
S652

刘文洪, 陈集双, 李永伟. 侵染天南星科植物病毒的分子鉴定及其生态学研究[J]. 应用生态学报, 2004, (4): 566-570.

LIU Wenhong, CHEN Jishuang, LI Yongwei. Molecular identification of Araceae-infecting viruses and their ecology[J]. Chinese Journal of Applied Ecology, 2004, (4): 566-570.

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