温度对转基因741杨抗虫性的影响

应用生态学报 ›› 2004, Vol. ›› Issue (5): 853-855.

温度对转基因741杨抗虫性的影响

刘军侠^1,2, 高宝嘉¹, 张炬红¹, 王永芳³, 王进茂¹

1. 河北农业大学, 保定 071000;
2. 东北林业大学, 哈尔滨 150040;
3. 河北省农林科学院谷子研究所, 石家庄 050031

收稿日期:2002-10-12 修回日期:2003-02-28 出版日期:2004-05-15
通讯作者: 高宝嘉
基金资助:
国家自然科学基金资助项目(30070626)

Influence of temperature on insect-resistance of transgenic hybrid poplar 741.

LIU Junxia^1,2, GAO Baojia¹, ZHANG Juhong¹, WANG Yongfang³, WANG Jinmao ¹

1. Hebei Agricultural University, Baoding 071000, China;
2. Northeast Forestry University, Harbin 150040, China;
3. Institute of Millet, Academy of Agricultural and Forest Sciences of Hebei Province, Shijiazhuang 050031, China

Received:2002-10-12 Revised:2003-02-28 Online:2004-05-15

摘要/Abstract

摘要： 在不同温度下测定转基因741杨的抗虫性,发现转抗虫基因741杨的抗虫性与温度密切相关.随着温度的升高,舞毒蛾幼龄幼虫的总死亡率、累计死亡率均明显升高,且随着昆虫龄级的增加,幼虫对转基因株系和温度的敏感性逐渐降低.温度对取食对照株系的昆虫生长发育有促进作用.温度高,舞毒蛾幼虫的生长发育明显加快,历期缩短,各龄期体重、排粪量增长快,而以转基因杨饲喂的昆虫发育进度随温度变化差异不显著.对1998～2000年温度资料和相应的杨扇舟蛾死亡率建立了回归方程,各高抗和中抗株系的温度昆虫死亡率曲线均表现为明显的回归关系.

关键词: 转基因741杨, 舞毒蛾, 抗虫性

Abstract: The study showed that the insect-resistance of transgenic hybrid poplar 741 was closely related with temperature.The total mortality and accumulative mortality of Gypsy moth (Lmantriadispar L.) larvae increased with increasing temperature,and the sensitivity of larvae to the clones of transgenic hybrid poplar 741 and temperature decreased gradually with the increasing instars.Temperature had a promotion to the development of larvae fed on CK.When the temperature was high,the development of larvae quickened,the development period shortened,and the weight increasing rate and the frass increasing quantity quickened,but the development rate of larvae fed on transgenic hybrid poplar 741 was not obvious with varied temperature.The regressive equation of temperature and Clostera anachoreta(Fabricius) mortality during 1998～2000 year indicated that high resistant and medium resistant clones' temperature-insect mortality curve presented an obvious regressive relation.

Key words: Transgenic hybrid poplar 741, Gypsy moth, Insect-resistance

中图分类号:

S763.42

刘军侠, 高宝嘉, 张炬红, 王永芳, 王进茂. 温度对转基因741杨抗虫性的影响[J]. 应用生态学报, 2004, (5): 853-855.

LIU Junxia, GAO Baojia, ZHANG Juhong, WANG Yongfang, WANG Jinmao . Influence of temperature on insect-resistance of transgenic hybrid poplar 741.[J]. Chinese Journal of Applied Ecology, 2004, (5): 853-855.

参考文献

[1] Alstsd DN,Anow DA.1995.Managing the evolution of inset resistance to transgenic plants.Science,268(30):1894～1896
[2] Benedict JH,Altman DW,Umbeck PF,et al.1992.Transgenic Bt cottons.J Econ Ent,85(4):1516～1521
[3] Creechio C,Stotzky G.1998.Insecticidal activity and biodegrade ton of the toxin from Bt bound to humic acids from soil.Soil Biol Biochern,30(4):463～470
[4] Ding Z-Y(丁志勇),Xu C-R(许崇任),Wang R-J(王戎疆).2001.Comparison of several important is enzymes between Bt cotton and regular cotton.Acta Ecol Sin(生态学报),21(2):332～336(in Chinese)
[5] Klopeensten NB.1993.Transfornation of populous hybrid to study and improve pest resistance.Silvae Cenet,42(2):86～90
[6] Li M-L(李明亮),Zhang H(张辉),Hu J-J(胡建军),et al.2002.Study on insect-resistant transgenic poplar plants containing both Bt and PI gene.Sci Sivae Sin(林业科学),36(2):93～96(in Chinese)
[7] Luo M-H(罗梅浩),Yang T-Z(杨铁钊),Ding Y-L(丁永乐),et al.1999.On the resistance of Bt and Cp TI dually transgenic tobacco to larvae of oriental tobacco budworm and blank cutworm.Acta PhloeylSin(植物保护学报),26(3):225～229(in Chinese)
[8] Sanders P.1998.Insect-resistant transgenic maize and security.Novenyvedelem,34(5):263～266
[9] Stark DM.1997.Risk assessment and criteria for commercial launch of transgenic plants,dialogue on risk assessrment of transgenic plants:Scien tific,technological and societal perspectives.Switlerland,28:21～35
[10] Tian Y-C(田颖川),Zheng J-B(郑均宝),Yu H-M(虞红梅),et al.2000.Studies of transgenic hybrid Poplar 741 carrying two insect-resistant genes.Acta Bot Sin(植物学报),42(3):263～268(in Chinese)
[11] Wei W(魏伟),Qga Y-Q(钱迎倩),Ma K-P(成克平).1999.Gene flow between transgenic crops and their wild related species.Acta Bot Sin(植物学报),41(4):343～348(in Chinese)
[12] Wei W(魏伟),Qian Y-Q(钱迎倩),Ma K-P(马克平).1999.Pests resistance to tranagenic Bt cope and their management strategies.Chin J Appl Environ Biol(应用与环境生物学报),5(2):215～222(in Chinese)
[13] Zhang Y-J(张永军),Wu K-M(吴孔明),Guo Y-Y(郭予元).2001.On the spatio-temporal expression of the contents of Bt insecticidal protein and the resistance of Bt transgenic cotton to cotton bollworm.Acta Phytophyl Sin(植物保护学报),28(1):1～6(in Chinese)
[14] Zhao K-J(赵奎军),Zhao J-Z(赵建周),Fan X-L(范贤林),et al.2000.Temporal and spatial dynamics of transgenic Bt cotton toxins to cotton bollworm in north China.J Agric Biotechnol(农业生物技术学报),8(1):49～52(in Chinese)
[15] Zherg J-B(郑均宝),Liang H-Y(梁海永),Gao B-J(高宝嘉),et al.2000.Selection and insect resistance of transgenic hybrid poplar 741 carrying two insect-resistant genes.Sci Silvae Sin(林业科学),36(2):93～97(in Chinese)