ARDRA对植物根瘤内共生放线菌Frankia多样性的研究

摘要/Abstract

摘要： 应用原核生物16S rDNA特异性引物rD1和fD1,通过ARDRA(amplified ribosomal DNA restrictionanalysis)法直接扩增自中国云南、东北地区赤杨属3种植物和沙棘属1种植物根瘤内Frankia DNA,得到一长约1500bp的扩增产物.选用两种内切酶HaeⅢ、AfaⅠ联合对扩增产物进行酶切,得到稳定的酶切图谱.将所测8个感染赤杨的Frankia样本区分为3个不同的组,所测3个感染沙棘的Frankia样本区分为3个不同的组.显示根瘤内Frankia存在丰富的遗传多样性.

关键词: 赤杨, 沙棘根瘤, Frankia, 多样性, ARDRA

Abstract: S ribosomal DNAs(rDNAs) of total 91 samples of Frankia strains in root nodules from Alnus and Hippophae were ampilified by PCR with primer rD1 and fD1.A fragment about 1500bp long was obtained.The amplicons were digested by HaeⅢ and Afa Ⅰ together and run in agarose gels.As a result,48 Alnus-infected Frankia were distinguished into three different groups and 43 Hippophae-infected Frankia into three different groups,respectively.The results showed that there is great genetic diversity among the Frankia strains directly from root nodules.

Key words: Alnus, Hippophae nodule, Frankia, Diversity, ARDRA

中图分类号:

Q939.13

吴少慧, 张惠文, 熊智, 刘忠, 张忠泽, 张成刚. ARDRA对植物根瘤内共生放线菌Frankia多样性的研究[J]. 应用生态学报, 2001, (6): 883-886.

WU Shaohui, ZHANG Huiwen, XIONG Zhi, LIU Zhong, ZHANG Zhongze, ZHANG Chenggang. Biodiversity of Frankia strains in nodules from Alnus and Hippophae by ARDRA[J]. Chinese Journal of Applied Ecology, 2001, (6): 883-886.

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