连续培养技术在微生态学研究中的应用

摘要/Abstract

摘要： 对连续培养技术在微生态学研究中的应用及进展作了综述.连续培养技术可用来建立体外模型以模拟正常菌群的生态系统,如人或动物的肠道菌群、口腔菌群等;用来研究正常菌群与病原菌之间的相互作用,菌群的生理生化特性及代谢产物.并可用于微生态制剂的研制和生产.

关键词: 连续培养, 正常菌群, 微生态学, 土壤养分, 生态保育, 野果林, 化学计量学, 环境因子

Abstract: The application of continuous flow culture in microbial ecology and its advances were reviewed.Continuous flow culture can be used to build in vitro models simulating ecosystems composed of normal bacteria such as human or animals intestinal and buccal bacteria,to examine the interactions between normal bacteria and pathogens,to investigate the physiological and biochemical traits of bacteria and their metabolic products,and to develop microecological preparations.

Key words: Continuous flow culture, Normal bacteria, Microecology, soil nutrient, environmental factor, stoichiometry, wild fruit forest, ecological conservation.

中图分类号:

Q938.1

曹郁生. 连续培养技术在微生态学研究中的应用[J]. 应用生态学报, 2002, (5): 629-631.

CAO Yusheng . Application of continuous-flow culture in microecological research[J]. Chinese Journal of Applied Ecology, 2002, (5): 629-631.

参考文献

[1] Basson NJ,Van Wyk CW.1996.The establishment of a community of oral bacteria that controls the growth of Candida albicans in a chemostat.Oral Microbiol and Immunol,11(3):199～202
[2] Bearne CA,Mallett AK,Rowland IR.1990.In:Parish WE,Hard GC eds.Proceeding of the Second International Conference in vitro Toxicology.522～525
[3] Bernhardt H,Wollmer A,Zimmormann K et al.1995.Growth of Candida albicans in normal and altered fecal flora in the model of continuous flow culture.Mycoses,38(suppl.1):22～27
[4] Corrier DE,Hinton AJR,Ziprin RL,et al.1990.Effect of dietary lactose on cecal pH,bacteriostatic volatile fatty acids,and Salmonella typhimurium colonization of broiler chicks.Avian Dis,34:617～625
[5] Freter R,Stauffer E,Cleven D,et al.1983.Continuous-flow cultures as in vitro models of the ecology of large intestinal flora.Infection and Immunity,39(2):666～675
[6] Greenman J,Holland KT.1985.Effects of dilution rate on biomass and extracellular enzyme production by three species of cutaneous Propionibacteria grown in continuous culture.J Gen Microbiol,131:1619～1624
[7] Hentges DJ,Freter R.1962.In vivo and in vitro antagonism of intestinal bacteria against Shigella flexneri Ⅰ.Correlation between various test.J Infect Dis,110:30～37
[8] Hillman K,Murdoch TA,Spencer RJ,et al.1994.Inhibition of enterotoxigenic Escherichia coli by the microflora of the porcine,in an in vitro semicontinuous culture system.J Appl Bacteriol,76:294～300
[9] Hume ME,Nisbet DJ,Deloach JR.1997.In vitro 14C-amino acid fermentation by CF3TM,a characterized continuous-flow competitive exclusion culture of caecal bacteria.J Appl Microbiol,83(2):236～242
[10] Keevil CW,Major NC,Davies DB,et al.1986.Physiology and virulence determinants of Neisseria gonorrhoeae growth in glucose-,oxygen-or cystine-limited continuous culture.J Gen Microbiol,132:3289～3302
[11] Maier BR,Onderdonk AB,Russell MS,et al.1972.Shigella,indigenous flora interactions in mice.Am J Clin Nut,25:1433～1440
[12] Mauchline WS,Araujo R,Wait R,et al.1992.Physiology and morphology of Legionella pneumophilla in continuous culture at low oxygen concentration.J Gen Microbiol,138:2371～2380
[13] Melville SB,Michael TA,Macy JM.1988.Pathway and sites for energy conservation in the metabolism of glucose by Selenomonos ruminantium.J Bacteriol,170:5298～5304
[14] Nisbet DJ,Corrier DE,Deloach JR.1993.Effect of mixed cecal microflora maintained in continuous culture and of dietary lactose on Salmonella typhimurium colonization in broiler chicks.Avian Diseases,37:528～536
[15] Nisbet DJ,Corrier DE,Rick SC,et al.1996.Maintenance of the biological efficacy in chicks of a caecal competitive-exclusion culture against salmonella by continuous-flow fermentation.J Food Protect,59(12):1279～1283
[16] Ouellette V,Chevalier P,Lacroix C.1994.Continuous fermentation of supplemented milk with immobilized Bifidobacterium infantis.Biotechnol Techni,8(1):45～50
[17] Silley P,Armstrong DG.1984.Changes in metabolism and cell size of the anaerobic bacterium Selenomonas ruminantium 0078A at the onset of growth in continuous culture.J Appl Bacteriol,56:487～492
[18] Sodini-Gallot I,Gorrieu G,Boquien CY,et al.1993.Process performance of continuous inoculation and acidification of milk with immobilized lactic acid bacteria.J Dairy Sci,78(7):1407～1420
[19] Tempest PW.1970.Methods in Microbiology Vol 2.London and New York:Academic Press.259～327
[20] Veilleux BG,Rowland I.1981.Simulation of the rat intestinal ecosystem using a two-stage continuous culture system.J Gen Microbiol,123:103～115
[21] Weimer PJ,Yan Shi,Odt CL.1991.A segment gas/liquid delivery system for continuous culture of microorganisms on insoluble substrates and its use for growth of Ruminococcus flavefaciens on cellulose.Appl Microbiol Biotechn,36:178～183
[22] Wilson KH,Freter R.1986.Interaction of Clostridium difficile and Escherichia coli with microfloras in continuous-flow cultures and gnotobiotic mice.Infection and Immunity,54(2):354～358
[23] Yan S,Weimer PJ.1992.Response surface analysis of the effects of dilution rate and pH on Ruminococcus flavefaciens FD-1 in cellulose-fed continuous culture.Appl Environ Microbiol,50:2583～2591