陈化烟叶中氨化细菌鉴定及有机氮降解特性

生态学杂志

陈化烟叶中氨化细菌鉴定及有机氮降解特性

周家喜¹,张晓敏²,胡大鸣²,惠建权²,余涛¹,邹晓^1*

(¹贵州大学生命科学学院，贵阳 550025； ^2贵州中烟工业有限责任公司，贵阳550025)

出版日期:2016-11-10 发布日期:2016-11-10

Isolation and identification of ammonifying bacteria in the flue-cured tobacco and characteristics of degrading organic nitrogen.

ZHOU Jia-xi¹, ZHANG Xiao-min², HU Da-ming², HUI Jian-quan², YU Tao¹, ZOU Xiao^1*#br#

(¹ Institute of Fungal Resource, College of Life Sciences, Guizhou University, Guiyang 550025, China; ² Guizhou Tobacco Industry Limited Liability Company, Guiyang 550025, China).

Online:2016-11-10 Published:2016-11-10

摘要/Abstract

摘要：

采用富集培养分离法，从烟样湖南A₃B₁（2013）、云南C₃F（2013）中分离出3株氨化细菌，编号GZUIFR-YC01、GZUIFR-YC02、GZUIFR-YC03。通过形态特征、生理生化特性和基于16S rDNA的系统学分析，鉴定菌株。应用纳氏试剂显色法初步研究3株菌的氨化能力。结果表明：菌株GZUIFR-YC01为嗜麦芽寡养单胞菌（Stenotrophomonas maltophilia），GZUIFR-YC02为纺锤形赖氨酸芽孢杆菌（Lysinibacillus fusiformis），GZUIFR-YC03为副短短芽孢杆菌（Brevibacillus parabrevis）。菌株GZUIFR-YC02长势优于菌株GZUIFR-YC01和GZUIFR-YC03，同时除氮能力明显高于其他两菌株。研究结果可为烟叶醇化生态过程中微生物驱动的氮元素转化提供参考。

关键词: 堆肥, 根区环境, 蔬菜残株, 日光温室, 蔬菜产量

Abstract: During a survey of ammonifying bacteria, three highactivity strains of ammonifying bacteria, viz., GZUIFR-YC01, GZUIFR-YC02, and GZUIFR-YC03, were screened from flue-cured tobacco (Hunan A₃B₁ and Yunnan C₃F produced in 2013) after enrichment culturing. Based on the morphological, physiological and biochemical characteristics and phylogenetic analysis, GZUIFR-YC01, GZUIFR-YC02, and GZUIFR-YC03 were identified as Stenotrophomonas maltophilia, Lysinibacillus fusiformis, and Brevibacillus parabrevis, respectively. In addition, L. fusiformis (GZUIFR-YC02) grew better than S. maltophilia (GZUIFR-YC01) and B. parabrevis (GZUIFR-YC03). While each of the three strains had a strong ability to degrade organic nitrogen, L. fusiformis (GZUIFR-YC02) was the best. This study provides reference to nitrogen transformation by microbes during the progress of tobacco leaf aging.

Key words: vegetable residue, solar greenhouse, vegetable yield., compost, root environment

周家喜1,张晓敏2,胡大鸣2,惠建权2,余涛1,邹晓1*. 陈化烟叶中氨化细菌鉴定及有机氮降解特性[J]. 生态学杂志.

ZHOU Jia-xi1, ZHANG Xiao-min2, HU Da-ming2, HUI Jian-quan2, YU Tao1, ZOU Xiao1*. Isolation and identification of ammonifying bacteria in the flue-cured tobacco and characteristics of degrading organic nitrogen.[J]. cje.

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