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盐渍化土壤翅碱蓬根际促生细菌的筛选及对多环芳烃的降解特性

宋立超1,钮旭光1,张玉龙1*,刘宛2,李培军2   

  1. (1沈阳农业大学土地与环境学院, 沈阳 110866; 2中国科学院沈阳应用生态研究所, 沈阳 110016)
  • 出版日期:2016-01-10 发布日期:2016-01-10

Screening of a plant growth-promoting rhizobacterium from Suaeda salsa (L.) in saline soil and its ability to degrade polycyclic aromatic hydrocarbons.

SONG Li-chao1, NIU Xu-guang1, ZHANG Yu-long1*, LIU Wan2, LI Pei-jun2   

  1. (1College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China; 2Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China)
  • Online:2016-01-10 Published:2016-01-10

摘要: 为了强化多环芳烃污染盐渍化土壤的原位植物-微生物修复的应用,获得促进植物降解多环芳烃的高效菌种资源,本研究采用富集培养的方法,从多环芳烃污染的大港油田翅碱蓬根际分离到1株能以菲、芘为唯一碳源同时分泌1-氨基环丙烷-1-脱氨酶的优势菌株B-1,通过形态观察和16S rRNA序列分析鉴定该菌株,并对其潜在的促生能力和菲、芘的降解特性进行分析。16S rRNA序列分析结果表明,该菌株为动性球菌属(Planococcus sp.),可产生3-吲哚乙酸,且具有溶磷能力。同时对菲、芘的降解具有较广泛的pH值和盐浓度范围,在菲和芘浓度均为50 mg·L-1,pH 8.0,盐度2%时,7 d对菲、芘的降解率为66.6%和52.0%。表面活性剂烷基糖苷的添加使菲、芘降解效率分别提高至94.2%和78.8%。

关键词: 气体交换, 遮荫, 耐冬山茶, 干旱

Abstract:

In order to apply the microbial-phytoremediation for the treatment of saline soil contaminated by polycyclic aromatic hydrocarbons (PAHs), the PAHs-specific degrading bacteria were studied in this paper. Phenanthrene and pyrene were used as carbon and energy sources, and one dominant strain (B-1) producing 1-aminocyclopropane-1-carboxylate (ACC) deaminase was isolated by enrichment culture from the PAHs-contaminated soil at Dagang Oilfield. The morphology and 16S rRNA gene sequencing analysis revealed that this strain belonged to Planococcus sp., and it could produce indol-3-ylacetic acid and dissolve phosphates. With phenanthrene and pyrene concentrations both at 50 mg·L-1, pH at 8.0 and salinity at 2%, their 7-day rates of degradation by strain B-1 were 66.6% and 52.0%, respectively. These percentages were significantly increased to 94.2% and 78.8% when alkyl polyglycoside was added into the system.
 

Key words: drought, shading, Camellia japonica (Naidong), gas exchange.