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土壤环境中转基因植物重组DNA持留与水平转移研究进展

吴元凤1,李刚1,冀国桢1,2,宋晓龙1,赵建宁1,杨殿林1,修伟明1,2**   

  1. (1农业部产地环境质量重点实验室,  农业部环境保护科研监测所, 天津 300191; 2沈阳农业大学植物保护学院, 沈阳 110866)
  • 出版日期:2015-03-10 发布日期:2015-03-10

Research progress of persistence and horizontal gene transfer of recombinant DNA from genetically modified plants in soil environment.

WU Yuan-feng1, LI Gang1, JI Guo-zhen1,2, SONG Xiao-long1, ZHAO Jian-ning1, YANG Dian-lin1, XIU Wei-ming1,2**   

  1. (1Key Laboratory of Original Agroenvironment Quality of Ministry of Agriculture, AgroEnvironmental Protection Institute, Ministry of Agriculture, Tianjin 300191, China; 2College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China)
  • Online:2015-03-10 Published:2015-03-10

摘要:

基因水平转移(horizontal gene transfer, HGT)是转基因植物环境风险评估的重要内容之一。转基因植物重组DNA通过根系分泌、花粉、残体等方式向土壤环境释放。已有研究表明,外源重组DNA很可能被土壤微生物通过同源重组的方式整合到基因组中,直接或间接地造成微生物群落结构和功能的改变,这将造成土壤生态环境系统的改变。本文论述了转基因植物重组DNA在土壤环境中的持留、水平转移及其影响因素和相关检测方法,讨论了转基因植物重组DNA在土壤环境中持留和水平转移的研究重点,并对其研究方法进行比较分析,提出今后的重点研究方向和方法,以期为转基因植物风险评估和安全管理提供技术支撑。

 

关键词: 干沉降速率, 生源要素, 干/湿沉降通量, 生态效应, 胶州湾

Abstract: Horizontal gene transfer (HGT) is an important part of the environmental risk assessment of genetically modified plants. Exogenous recombinant DNA can be released from transgenic plants into the soil environment through root exudates, pollen, debris, etc. Studies have shown that the recombinant DNA could be integrated into the genome of soil microorganisms by homologous recombination. This would have direct or indirect effects on the structure and function of soil microbial community, and even result in the changes of soil ecosystem. This article focused on the persistence and HGT of the recombinant DNA in soil environment and the influence factors and detection methods, discussed the research highlights of the persistence and HGT of recombinant DNA, compared the research methods, and proposed the focus of future research in order to provide technical support for the risk assessment and security management of genetically modified plants.

Key words: atmospheric dry deposition velocity, ecological effects, biogenic elements, fluxes of dry and wet depositions, Jiaozhou Bay