松口蘑(Tricholoma matsutake)菌塘土壤可培养细菌多样性

生态学杂志

松口蘑(Tricholoma matsutake)菌塘土壤可培养细菌多样性

姜华^1,2,何承刚²,于富强^1**,刘培贵¹,赵文青¹

(¹中国科学院昆明植物研究所生物多样性与生物地理学重点实验室，昆明 650201； ^2云南农业大学动物科学技术学院，昆明 650201)

出版日期:2015-01-10 发布日期:2015-01-10

Bacterial diversity cultured from shiros of Tricholoma matsutake

JIANG Hua^1,2, HE Cheng-gang², YU Fu-qiang^1**, LIU Pei-gui¹, ZHAO Wen-qing^1

(¹Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; ²Faculty of Animal Sciences and Technology, Yunnan Agricultural University, Kunming 650201, China)

Online:2015-01-10 Published:2015-01-10

摘要/Abstract

摘要： 松口蘑(Tricholoma matsutake)也称松茸，是具有重要经济和药用价值的野生食用菌，菌塘是其子实体发生发育的场所。本文采用土壤平板稀释技术研究了云南省6份松口蘑菌塘土壤可培养细菌，共获得了178条细菌的16S rDNA碱基序列，经分析分别属于4个门、18个属、38个OTUs，变形菌门(Proteobacteria)序列占58.43%，厚壁菌门(Firmicutes)占26.40%，拟杆菌门(Bacteroidetes)占10.67%，放线菌门(Actinobacteria)占4.49%，其中厚壁菌门的芽孢杆菌属(Bacillus)占24.72%，变形菌门的伯克氏菌属(Burkholderia)占21.34%，假单胞菌属(Pseudomonas)占11.24%，拟杆菌门的金黄杆菌属(Chryseobacterium)占10.67%。云南松口蘑菌塘土壤可培养细菌的多样性较为丰富。

关键词: 全膜双垄沟, 冠层覆盖度, AquaCrop模型, 施氮量, 籽粒产量

Abstract:

Matsutake (Tricholoma matsutake) is an important wild edible mushroom with important economic and medicinal value. The sporocarps often occur and develop in shiros. In this study, soils from six shiros of T. matsutake were collected in Yunnan Province and soil dilution plating was used to determine the diversity of bacterial species represented in these samples. Sequencing of 16S rDNA generated 178 sequences from cultured bacteria, representing 38 operational taxonomic units (OTUs) of 4 phyla and 18 genera. The majority of sequences belonged to Proteobacteria (58.43%), Firmicutes (26.40%), Bacteroidetes (10.67%), and Actinobacteria (4.49%). Further analysis revealed that bacteria cultured from these soils were species of Bacillus (Firmicutes, 24.72%), Burkholderia (Proteobacteria, 21.34%), Pseudomonas (Proteobacteria, 11.24%), and Chryseobacterium (Bacteroidetes, 10.67%). Therefore, the diversity of cultured bacteria from the shiros of matsutake in Yunnan Province was abundant.

Key words: AquaCrop model, canopy cover degrees, nitrogen application rate, whole plastic-film mulching on double ridges, grain yield

姜华1,2,何承刚2,于富强1**,刘培贵1,赵文青1. 松口蘑(Tricholoma matsutake)菌塘土壤可培养细菌多样性[J]. 生态学杂志.

JIANG Hua1,2, HE Cheng-gang2, YU Fu-qiang1**, LIU Pei-gui1, ZHAO Wen-qing1. Bacterial diversity cultured from shiros of Tricholoma matsutake[J]. cje.

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