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[OMIm]Br对蛋白核小球藻的致毒效应

杨兆壬,周作明**   

  1. (华侨大学环境科学与工程系, 福建厦门 361021)
  • 出版日期:2014-06-10 发布日期:2014-06-10

Toxic effect of \[OMIm\]Br on Chlorella pyrenoidosa.

YANG Zhao-ren, ZHOU Zuo-ming**   

  1. (Department of Environmental Science & Technology, Huaqiao University, Xiamen 361021, Fujian, China)
  • Online:2014-06-10 Published:2014-06-10

摘要: 通过急性毒性实验考察了溴化1-辛基-3-甲基咪唑(\[OMIm\]Br)离子液体对蛋白核小球藻(Chlorella pyrenoidosa)的致毒作用。结果表明:经过\[OMIm\]Br处理96 h,藻细胞生物量(包括藻细胞密度、叶绿素和蛋白质含量)发生改变,呈现正相关的剂量-效应关系;\[OMIm\]Br抑制蛋白核小球藻生长的半最大效应浓度(EC50)为14.95 mg·L-1;藻细胞的抗氧化机制受到破坏,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性均下降,膜脂质过氧化产物丙二醛(MDA)的含量增加,且SOD、POD、MDA三者的变化幅度与受试物剂量呈正相关;\[OMIm\]Br通过直接损伤蛋白质和破坏抗氧化机制系统等途径对蛋白核小球藻产生致毒机制,运用流式细胞仪等手段证实藻细胞周期被\[OMIm\]Br阻滞在S期和G2/M期,并导致藻细胞坏死,阻滞程度和坏死程度与\[OMIm\]Br剂量呈正相关。

关键词: 肉桂酸, 花生, 土壤酶, 对羟基苯甲酸, 连作障碍, 产量, 土壤养分

Abstract: The toxicity of 1-octyl-3-methyimidazolium bromide (\[OMIm\]Br) ionic liquid on Chlorella pyrenoidosa was investigated by acute toxicity tests The results showed that under \[OMIm\] Br stress for 96 h, algal biomass (algal cell density, chlorophyll and protein contents) changed with the \[OMIm\]Br dosage in a positive correlation. The concentration for 50% of maximal effect (EC50) of \[OMIm\]Br to C. pyrenoidosa was 14.95 mg·L-1. The activities of superoxide dismutase (SOD) and peroxidase (POD) decreased, whereas the malondialdehyde (MDA) content increased with the increasing \[OMIm\]Br dosage. The direct harm of \[OMIm\]Br to protein and antioxidant system was the main cause of toxicity to C. pyrenoidosa. It was proved that, owing to the damage of \[OMIm\]Br revealed by flow cytometry (FCM) detection, C. pyrenoidosa cell cycle stopped at S phase and G2/M phase, and [OMIm]Br resulting in necrosis. The degree of cell retardation and necrosis was positively correlated with the \[OMIm\]Br dosage.

Key words: continuous cropping obstacle, soil enzyme, p-hydroxy benzoic acid, cinnamic acid, peanut, yield., soil available nutrients