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濒危植物扇脉杓兰野生居群遗传多样性的AFLP分析

钱鑫1,李全健1,2,连静静1,王彩霞1,田敏1**   

  1. (1中国林业科学研究院亚热带林业研究所, 浙江富阳 311400; 2中国科学院上海辰山植物科学研究中心, 上海 201602)
  • 出版日期:2013-06-10 发布日期:2013-06-10

Genetic diversity of endangered wild Cypripedium japonicum populations: An AFLP analysis.

QIAN Xin1, LI Quan-jian1,2, LIAN Jing-jing1, WANG Cai-xia1, TIAN Min1**   

  1. (1Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyan 311400, Zhejiang, China; 2Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai 201602, China)
  • Online:2013-06-10 Published:2013-06-10

摘要: 扇脉杓兰(Cypripedium japonicum)是一种珍稀的地生兰。为了探索并制定针对性的保护措施,采用AFLP分子标记技术对现存于浙江省的6个扇脉杓兰天然居群进行了遗传多样性和遗传结构的研究。筛选出的7对引物共扩增出377条清晰条带,多态性比率为20.95%。居群总Nei基因多样性指数为0.0512,其中居群内基因多样性为0.0247,居群平均Shannon多态性信息指数为0.0417,群体间总遗传分化系数为0.5178,基因流为0.4655,表明扇脉杓兰居群的遗传多样性较低,居群间出现一定程度的遗传分化。UPGMA聚类和Mantel检测分析结果表明,居群间的地理位置与遗传距离之间不存在显著相关性(r=0.16,P=0.242)。基于本研究结果并结合扇脉杓兰的生物学特性,初步提出了相应的保育策略。

关键词: 周年温室气体排放, 硝化抑制剂, 控释尿素, 稻田

Abstract: Cypripedium japonicum is a rare and endangered terrestrial orchid. To explore and formulate the pertinent conversation strategies of this species, this paper analyzed the genetic diversity and genetic structure of six natural C. japonicum populations in Zhejiang Province of East China by the method of AFLP. A total of 377 distinct bands were amplified by 7 pairs of selected primers. The percentage of polymorphic loci was 20.95%, Nei’s genetic diversity index was 0.0512, Shannon information index was 0.0417, genetic differentiation coefficient was 0.5178, and gene flow was 0.4655, indicating that there was a relatively low genetic diversity within the C. japonicum populations, and a certain degree of genetic divergence  existed among the populations. The analyses of UPGMA clustering and Mantel test showed that there was no significant correlation between the geographical distance and genetic distance (r=0.16, P=0.242). Some conservation strategies were proposed based on this study and the biological properties of C. japonicum.

Key words: annual greenhouse gas emission, nitrification inhibitor, paddy field., controlled release urea