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生态学杂志 ›› 2012, Vol. 31 ›› Issue (10): 2585-2590.

• 研究报告 • 上一篇    下一篇

北部湾6个拟穴青蟹群体遗传多样性的ISSR分析

宋忠魁1,2**,孙奉玉1,3,李梦芸1,4,赵鹏1,聂振平1,苏琼1   

  1. 1广西海洋研究所广西海洋生物技术重点实验室, 广西北海 536000; 2钦州学院化学与生物系, 广西钦州 535000; 3南华大学药学与生命科学学院, 湖南衡阳 421001; 4湖南农业大学生物科学技术学院, 长沙 410128)
  • 出版日期:2012-10-10 发布日期:2012-10-10

Genetic diversity of six mud crab (Scylla paramamosain) populations in Beibu Gulf of South China based on ISSR analysis.

SONG Zhong-kui1, 2**, SUN Feng-yu1, 3, LI Meng-yun1, 4, ZHAO Peng1, NIE Zhen-ping1, SU Qiong1   

  1. (1Guangxi Key Laboratory for Marine Biotechnology, Guangxi Institute of Oceanology, Beihai 536000, Guangxi, China; 2Department of Chemistry and Biology, Qinzhou University, Qinzhou 535000, Guangxi, China; 3Pharmacy and Life Science, South China University, Hengyang 421001, Hunan, China; 4College of Biology and Technology, Hunan Agricultural University, Changsha 410128, China)
  • Online:2012-10-10 Published:2012-10-10

摘要: 应用ISSR标记技术分析来自北部湾的拟穴青蟹6个地理群体(清化、党江、钦州湾、流沙湾、珍珠湾、闸口)的遗传变异和遗传结构,8条ISSR引物扩增111个个体,分析其中的66个位点,56个位点表现出多态性,表明拟穴青蟹在物种水平上的多态位点百分率是84.85%。拟穴青蟹6个群体的多态位点百分率为51.52%~63.64%,平均为57.58%。群体的遗传多样性按自高至低顺序排列为清化群体>党江群体>钦州湾群体>流沙湾群体>珍珠湾群体>闸口群体。拟穴青蟹在种水平或群体水平上的多态位点百分率和Shannon信息指数表明,拟穴青蟹遗传多样性在甲壳类动物中处于较高层次,但比甲壳类以外的几类经济海产动物遗传多样性低。总的遗传分化系数GST值为0.1841,表明,总遗传变异的绝大部分存在于群体内,群体间的遗传分化程度较大。但是,绝大部分两两群体间的遗传分化系数值在0.0689~0.1276,为中等程度的遗传分化。AMOVA分析表明,群体内遗传变异占87.97%,群体间遗传变异占12.03%,群体间遗传分化程度中等但分化显著。Mantel test结果表明,拟穴青蟹6个群体间的遗传距离与地理距离之间的相关性不显著。聚类分析表明,来自广西沿海的4个群体聚成一支。

关键词: 滨海盐土, 高光谱参数, 土壤水分, 高光谱遥感

Abstract: ISSR-PCR technique was adopted to investigate the genetic variation and genetic structure of six Scylla paramamosain geographic populations (QH, DJ, QZW, LSW, ZZW, and ZK) in the Beibu Gulf of South China. A total of 111 individuals were amplified by 8 ISSR primers, and 66 valid loci were analyzed, of which, 56 loci were polymorphic. The percentage of polymorphic loci (PPL) was 84.85% at species level. The PPL of the six populations ranged from 51.52% to 63.64%, with an average of 57.58%. The genetic diversity of the six populations decreased in the order of QH > DJ >QZW> LSW >ZZW >ZK. The PPL and the Shannon′s information index at species or population level showed that the genetic diversity of S. paramamosain arrived at a higher level within the crustacean, but was still lower than that of the other several marine animals of economic importance. The total coefficient of genetic differentiation (GST=0.1841) showed that most of the total genetic variation variance (81.59%) indwelled within populations, suggesting a higher level of genetic differentiation among the populations. However, the coefficient of genetic differentiation (GST=0.0689-0.1276) of pairwise populations suggested the mediumdegree genetic differentiation among the populations. The analysis of molecular variance (AMOVA) showed that the genetic variation variance within and among the populations accounted for 87.03% and 12.97%, respectively, suggesting that the genetic differentiation among the populations was at a medium level, but the differentiation was significant (P<0.001). The Mantel test suggested that there was no significant correlation between the genetic distance and geographic distance of the six S. paramamosain populations. Clustering analysis showed that the four populations from Guangxi coastal areas could be clustered into a clade.

Key words: coastal saline soils, hyperspectral parameters, soil moisture content, hyperspectral remote sensing.