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生态学杂志 ›› 2012, Vol. 31 ›› Issue (07): 1785-1790.

• 研究报告 • 上一篇    下一篇

DEHP对中国林蛙精巢中StAR、CYP17和CYP19基因表达的影响

李苗,张育辉**   

  1. (陕西师范大学生命科学学院, 西安 710062)
  • 出版日期:2012-07-10 发布日期:2012-07-10

Effects of di-2-ethylhexyl phthalate (DEHP) on the expression of StAR, CYP17, and CYP19 in Rana chensinensis testis.

LI Miao, ZHANG Yu-hui**   

  1. (College of Life Science, Shaanxi Normal University, Xi’an 710062, China)
  • Online:2012-07-10 Published:2012-07-10

摘要: 为探讨邻苯二甲酸二(2-乙基己基)酯(di-2-ethylhexyl phthalate,DEHP)对两栖动物精巢类固醇激素合成的影响,将中国林蛙(Rana chensinensis)雄性成体分别暴露于浓度为10-7、10-6、10-5、10-4 mol·L-1 DEHP的水体,分别在暴露20、30和40 d取其精巢,提取精巢总RNA,逆转录合成cDNA,通过荧光实时定量PCR检测StAR、CYP17和CYP19 mRNA表达相对值。结果表明:与对照组相比,DEHP处理组StARCYP19基因表达均上调,CYP17基因表达下调;比较不同DEHP浓度和不同暴露时间对StAR、CYP17和CYP19 mRNA表达相对值的影响,显示DEHP浓度变化对3个基因表达影响的规律性不强,而DEHP暴露时间的累积效应较明显;提示DEHP可通过干扰中国林蛙精巢中StAR、CYP17和CYP19基因表达,影响其相应关键酶的表达,从而干扰类固醇激素的合成,产生雌激素效应。

关键词: 潜流层, 大型无脊椎动物, 功能摄食类群, 垂直分布

Abstract: In order to investigate the effects of di-2-ethylhexyl phthalate (DEHP) on the steroid hormone synthesis in amphibian testis, the male adults of Rana chensinensis were exposed to 10-7, 10-6, 10-5 and 10-4 mol·L-1 of DEHP for 20, 30 and 40 days, respectively, and the total RNA in the testis was extracted, the cDNA synthesis was performed by reverse transcription, and the relative mRNA expression values of StAR,CYP17 and CYP19 were determined by fluorescence real-time quantitative PCR. As compared with the control, the expression of StAR and CYP19 was up-regulated, and that of CYP17 was down-regulated. The effects of different concentration DEHP on the mRNA expression of StAR, CYP17 and CYP19 were less regular, whereas the cumulative effect of exposure time was more obvious. It was suggested that DEHP could affect the expression of related key enzymes in Rana chensinensis  testis via interfering with the expression of StAR, CYP17 and CYP19 in the testis, and then, interfere with the synthesis of steroid hormones and result in estrogen effect.

Key words: hyporheic zone, macroinvertebrates, functional feeding group, vertical distribution.