欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志

• 研究报告 • 上一篇    下一篇

高环多环芳烃降解菌的筛选及其降解特性

高野萌1,杨雪莲1*,李凤梅2,王卅3,滕菲1,王武1,沈元昱1
  

  1. 1沈阳大学, 沈阳 110044; 2中国科学院沈阳应用生态研究所, 沈阳 110016; 3中国科学院大学, 北京 100049)
  • 出版日期:2016-06-10 发布日期:2016-06-10

Isolation of heavy PAH-degrading bacteria and their characteristics of degradation.

GAO Ye-meng1, YANG Xue-lian1*, LI Feng-mei2, WANG Sa3, TENG Fei1, WANG Wu1, SHENG Yuan-yu1#br#   

  1. (1 Shenyang University, Shenyang 110044, China; 2 Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 3 University of Chinese Academy of Sciences, Beijing 100049, China).
  • Online:2016-06-10 Published:2016-06-10

摘要: 通过富集培养及平板升华法从本溪钢铁公司周边多环芳烃(PAHs)污染土壤中分离出7株PAHs降解菌。以芘和苯并\[a\]芘为底物进行摇瓶降解实验,结果表明:G1、G2和G3菌株对高环PAHs芘和苯并\[a\]芘均具有较强的降解能力。进一步研究此3株菌及混合菌对原状污染土壤中PAHs的降解能力,发现80 d时对总PAHs的降解顺序依次为:混合菌>G2>G1>G3,其中混合菌对PAHs降解率较单菌分别提高了9.17%、11.49%和16.11%;4个处理对4~6环PAHs的降解率较对照组相比提高的倍数随着环数增加而增大;总PAHs的降解率与脱氢酶的活性呈正相关。电场影响G1、G2和G3菌株对PAHs降解,在1.0 V·cm-1电场条件下,4环、5环及6环PAHs降解率较单纯微生物修复提高12.13%、13.35%和14.52%,说明3株菌具有较强的电场适应能力,可在高环PAHs污染土壤的电动微生物修复中应用。形态学观察及16S rRNA序列比对分析表明,G1、G2、G3菌株分别为鞘氨醇单胞菌属(Sphingomonas sp.)、苍白杆菌属(Ochrobactrum sp.)和无色杆菌属(Achromobacter sp.)。

关键词: 花生, 钙肥, 干旱胁迫, 生理特性, 产量品质

Abstract: Seven PAH-degrading strains were isolated from PAHs-contaminated soil around an iron and steel company in Benxi using the enrichment procedure and tablet sublimation method. Their degradation ability of PAHs was studied in flasks with pyrene or benzo\[a\]pyrene as the single carbon source. The results showed that G1, G2 and G3 strains had stronger degradation ability for heavy PAHs. Further studies on the degradation ability of G1, G2, G3 and their mixture in PAHscontaminated soil indicated that at 80 days, the total PAH-degradation rates in the four treatments were in order of the mixture > G2 > G1 > G3. Compared to the treatments that inoculated with G1, G2 and G3 alone, the degradation rate in the treatment inoculated with the mixture was enhanced by 9.17%, 11.49%, and 16.11%, respectively. Compared with the control treatment, the degradation rates of 4-6-ring PAHs increased in the four treatments and the times of their increase were enhanced with the number of rings. Meanwhile, there was a positive correlation between the dehydrogenase activity and PAHs degradation. The degradation rates of 4-, 5-, and 6-ring PAHs were enhanced by 12.13%, 13.35%, and 14.52% in BioEK experiment than in the Bio experiment, which indicated that G1, G2 and G3 have good ability of adapting to electric field. G1, G2 and G3 strains were identified as Sphingomonas sp., Ochrobactrum sp. and Achromobacter sp. respectively based on their physiological characteristics and the sequence analysis of 16S rRNA.

Key words: peanut, calcium fertilizer, drought stress, physiological characteristics, yield and quality.