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茄子光系统Ⅱ的热胁迫特性

王梅1;高志奎1;黄瑞虹1;王惠英1;张文丽1;高荣孚2   

  1. 1河北农业大学园艺学院, 河北保定 071001;
    2北京林业大学生物科学与技术学院, 北京 100083
  • 收稿日期:2005-12-30 修回日期:2006-11-03 出版日期:2007-01-18 发布日期:2007-01-18

Heat stress characteristics of photosystem Ⅱ in eggplant

WANG Mei1;GAO Zhi-kui1;HUANG Rui-hong1;WANG Hui-ying1;ZHANG Wen-li1;GAO Rong-fu2   

  1. 1College of Horticulture, Hebei Agricultural University, Baoding 071001,Hebei, China;
    2College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
  • Received:2005-12-30 Revised:2006-11-03 Online:2007-01-18 Published:2007-01-18

摘要: 以耐热性较弱的黑贝一号圆茄和耐热性较强的黑贝二号圆茄为试材,热胁迫处理后采用植物效率仪PEA进行快速叶绿素荧光诱导曲线及其参数测定.结果表明:当温度高于40 ℃,PSⅡ结构受热胁迫影响较为敏感,表现为初始荧光Fo缓慢上升;PSⅡ原初光化学效率Fv/Fm和ΔF/Fm′大幅度下降,且黑贝二号Fv/Fm的半衰时间T50和ΔF/Fm′的半衰温度t50分别大于黑贝一号.较高的热胁迫剂量(48℃处理5 min或44℃处理20~30min)下,快速荧光诱导动力学曲线呈现OKJIP型,在700μs处出现与放氧复合体失活有关的K相.黑贝一号在44 ℃下处理20 min才有K相出现,黑贝二号则晚10 min出现.与35℃相比,在48℃,特别是在52℃的较高剂量热胁迫下,Strasser能量流动模型参数中的DIo/RC有大幅度地增加,体现了热耗散对PSⅡ的较强保护能力.随着热胁迫温度的升高和热胁迫时间的延长,两品种的无活性中心Fvi/Fv显著增加.

关键词: SO2, 时空分布, 被动采样, 酸雨, 杭州

Abstract: With lower-and higher heat-resistant varieties of eggplant (Solanum melongena L.) HeibeiⅠand Heibei Ⅱ as test materials, and by using Plant Efficiency Analyzer (PEA) from Hansatech, this paper measured the fast chlorophyll a fluorescence transient and its parameters. The results showed that PS Ⅱ construction became more sensitive to heat stress when ambient temperature was higher than 40 ℃. The Fo went up slowly, and Fv/Fm and ΔF/Fm′came down dramatically. Heibei Ⅱ had a longer semi-attenuation temperature of Fv/Fm (T50) and ΔF/Fm′(t50) than Heibei Ⅰ. Under strong heat stress (5 min at 48 ℃ or 20-30 min at 44 ℃), the K-step in relation to the inactivation of oxygen-evolving complex appeared in fluorescence rise at about 700 μs, and the regular O-J-I-P transient was transformed to O-K-J-I-P one. The K-phase of HeibeiⅠand Heibei Ⅱappeared when the treatment time was up to 20 and 30 minutes at 44 ℃, respectively. In comparing with 35 ℃heat treatment, the DIo/RC in the parameters of Strasser′s specific energy fluxes model was increased by a great extent under 48 ℃ or more heat stress, reflecting a strong safeguard of energy dissipation to PS Ⅱ. When the temperature of heat stress increased from 35 ℃ to 52 ℃, the Fvi/Fv of PS Ⅱ silent reaction centers of HeibeiⅠand Heibei Ⅱ increased remarkably.

Key words: SO2, Spatial temporal distribution, Passive sampling, Acid rain, Hangzhou