麻疯树根边缘细胞生物学特性及镉对其活性的影响

生态学杂志 ›› 2011, Vol. 30 ›› Issue (07): 1423-1428.

麻疯树根边缘细胞生物学特性及镉对其活性的影响

梁剑1,2,刘小文3,唐琳1,徐莺1,王胜华1,陈放1**

1四川大学生命科学学院生物资源与生态环境教育部重点实验室，成都 610064；2西昌学院农业科学学院，四川西昌 615013；3云南农业大学植物保护学院，昆明 650201

出版日期:2011-07-08 发布日期:2011-07-08

Biological characteristics of Jatropha curcas root border cells and toxic effect of cadmium on the cell viability.

LIANG Jian1,2, LIU Xiao-wen3, TANG Lin1, XU Ying1, WANG Sheng-hua1, CHEN Fang1**

1Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China; 2Institute of Agricultura Sciences, Xichang College, Xichang 615013, Sichuan, China; 3College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China

Online:2011-07-08 Published:2011-07-08

摘要/Abstract

摘要： 以麻疯树为试材，采用悬空气法，探讨麻疯树离体根边缘细胞的生物学特性及重金属镉(Cd²⁺)对根边缘细胞活性的影响。结果表明,麻疯树根边缘细胞大多数呈椭圆型，少数成弯曲的长条型；根长为15 mm时，边缘细胞的生物学活性（存活率）最大；根冠果胶甲基脂酶（PME）酶活性随着根的伸长有所降低。镉对麻疯树根边缘细胞产生明显毒害，随着镉浓度和处理时间的增加，边缘细胞的活性都呈现下降的趋势。

关键词: 生态有机肥, 番茄青枯病, FAMEs, 土壤微生物多样性

Abstract: Jatropha curcas root border cells were detached from root tip and cultured by aeroponics to study their biological characteristics and the toxic effect of cadmium on their viability. Most of the cells were elliptical, and a few of them presented bend claval shape. The cells had the highest viability when the
root length was 15 mm, and their pectin methylesterase (PME) activity somewhat decreased with root elongation. Cadmium had obvious toxic effect on the cells. With the increasing concentration of cadmium and the increasing treating time, the viability of the border cells all showed a decreasing trend.

Key words: Ecological organic fertilizer, Bacterial wilt of tomato, FAMEs, Soil microbial diversity

梁剑,刘小文,唐琳,徐莺,王胜华,陈放. 麻疯树根边缘细胞生物学特性及镉对其活性的影响[J]. 生态学杂志, 2011, 30(07): 1423-1428.