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生态学杂志 ›› 2011, Vol. 30 ›› Issue (03): 448-452.

• 研究报告 • 上一篇    下一篇

刺萼龙葵不同生育期根际土壤酶活性和真菌多样性变化

祝明炜1;曲波1**;杨红2;陈旭辉1;王承旭1;付卫东3;张国良3   

  1. 1沈阳农业大学生物科学技术学院, 沈阳 110161;2沈阳农业大学植物保护学院, 沈阳 110161;3中国农业科学院农业环境与可持续发展研究所, 北京 100081
  • 出版日期:2011-03-08 发布日期:2011-03-08

Changes of soil enzyme activities and fungal diversity in rhizosphere soil of Solanum rostratum at different development stages.

ZHU Ming-wei1, QU Bo1**, YANG Hong2, CHEN Xu-hui1, WANG Cheng-xu1, FU Wei-dong3, ZHANG Guo-liang3   

  1. 1College of Biological Technology, Shenyang Agricultural University, Shenyang 110161, China|2College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China|3Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Online:2011-03-08 Published:2011-03-08

摘要: 分析入侵植物刺萼龙葵(Solanum rostratum)在不同生育期根际土壤真菌多样性及土壤酶活性变化,探讨刺萼龙葵入侵对土壤微生态的影响机制,以期寻找防治刺萼龙葵的最佳时期。本试验利用土壤平板法对刺萼龙葵根际土壤真菌进行培养,共鉴定真菌22个属,并且从生长初期到开花期土壤真菌多样性显著增加;从生长初期到开花期根际土壤过氧化氢酶、过氧化物酶、多酚氧化酶、脲酶、脱氢酶、转化酶和蛋白酶活性变化显著;Pearson相关分析发现,刺萼龙葵从生长初期到开花期根际土壤真菌多样性与过氧化物酶活性相关性并不显著,而与其余6种土壤酶活性具有显著的相关性;主成分分析发现,刺萼龙葵开花期、现蕾期和四叶期与空白土壤和生长初期发生了显著的变化。因此,随着刺萼龙葵的生长,其根际土壤真菌多样性及土壤酶活性均发生改变。

关键词: 种子库, 空间格局, 尺度, 水曲柳

Abstract: In order to understand the effects of Solanum rostratum invasion on soil micro-ecosystem, and to approach the optimum time of controlling this invasion, this paper studied the soil enzyme activities and fungal diversity in the rhizosphere soil of S. rostratum at its different development stages. Plate method was applied to culture the fungi, and titration method and colorimetric method were adopted to determine the activities of soil catalase, peroxidase, polyphenol oxidase, urease, desaturase, invertase, and protease. A total of 22 genera fungi were identified. From the early growth stage to flowering stage of S. rostratum, the fungal diversity increased significantly, and the enzyme activities varied obviously. Pearson analysis showed that there existed significant relationships between soil fungal diversity and the six soil enzyme activities except for peroxidase from the early growth stage to flowering stage, and principal component analysis showed that the fungal diversity and enzyme activities at the flowering, budding, and four-leaf stages had significant differences with those at the early growth stage of S. rostratum and in the control soil. Therefore, with the growth of S. rostratum, the fungal diversity and enzyme activities in rhizosphere soil changed.

Key words: Seed bank, Spatial pattern, Scales, Fraxinus mandshurica