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生态学杂志 ›› 2009, Vol. 28 ›› Issue (09): 1818-1823.

• 研究报告 • 上一篇    下一篇

缺硫对万寿菊镉积累的影响

冯倩1,2;付莎莎1,2;台培东1**;李培军1;张海荣1;张银秋1,2;陈扬1,2,3   

  1. 1中国科学院沈阳应用生态研究所|沈阳 110016|2中国科学院研究生院|北京 100039|3中国科学院高能物理研究所|北京 100049
  • 出版日期:2009-09-10 发布日期:2009-09-10

Cadmium accumulation in Tagetes erecta as affected by sulfur deficiency.

FENG Qian1,2;FU Sha-sha1,2;TAI Pei-dong1; |LI Pei-jun1|ZHANG Hai-rong1;ZHANG Yin-qiu1,2|CHEN Yang1,2,3   

  1. 1Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China|2Graduate University of Chinese Academy of Sciences, Beijing 100039, China|3Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
  • Online:2009-09-10 Published:2009-09-10

摘要: 采用营养液培养法,研究了缺硫对万寿菊(Tagetes erecta)各器官镉积累的影响,以及巯基化合物和有机酸等在各器官镉积累中的作用。结果表明:0.1 mg·L-1镉处理7 d,缺硫处理显著降低了植物根、茎和叶中的镉含量,较对照分别降低了47%、38%和61%;缺硫对植物体内半胱氨酸、γ谷氨酰半胱氨酸、谷胱甘肽和有机酸的合成没有显著影响,而且3种巯基化合物的含量都很低;缺硫植物根部合成的植物络合素的量显著降低,这可能是植物体内镉积累减少的主要原因;在植物的各器官中,叶片中的有机酸含量最高,而在叶片中未检测到植物络合素,由此可推断,片中积累的镉主要与有机酸结合;缺硫影响了根部植物络合素的合成,进而降低了植物体内镉的积累,而缺硫没有影响植物体内有机酸的合成,有机酸可能仅是参与植物组织中与镉的络合作用。

关键词: 苔藓, 培养基, 光照培养, 激素

Abstract: A hydroponic culture was conducted to study the cadmium (Cd) accumulation in Tagetes erecta as affected by Sdeficiency, and to evaluate the roles of thiol compounds and organic acids in the Cd accumulation. After exposure to 0.1 mg Cd·L-1 for 7 days, the Cd content in T. erecta root, stem, and leaf under S-deficiency decreased significantly by 47%, 38%, and 61%, respectively, compared with the control. S-deficiency had no significant effects on the synthesis of cysteine, γ-glutamyl cysteine, glutathione, and organic acids, and the three thiol compounds were all in lower levels. The synthesized phytochelatins (PCs) amount in S-deficient T. erecta root decreased significantly, which could be the main cause of the reduction of Cd accumulation in the plant. Among the plant organs, leaf had the highest organic acids content but no PCs, suggesting that the accumulated Cd in leaf was mainly bound by organic acids. All the results indicated that S-deficiency affected the synthesis of PCs in root, inducing the decrease of Cd accumulation in plant, but had less effects on the synthesis of organic acids that could possibly participate in the chelation of Cd in plant tissues.

Key words: Moss, Culture medium, Light culture, Growth hormone