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生态学杂志 ›› 2009, Vol. 28 ›› Issue (10): 2032-2036.

• 专论与综述 • 上一篇    下一篇

美国白蛾种群遗传分化的RAPD分析

阚国仕1**;李 雪1;丁建云2   

  1. 1沈阳农业大学生物科学技术学院| 沈阳 110161;2北京市植物保护站| 北京 100029
  • 出版日期:2009-11-10 发布日期:2009-11-10

RAPD analysis on genetic differentiation of Hyphantria cunea populations.

KAN Guo-shi1;LI Xue1;DING Jian-yun2   

  1. 1College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110161, China|2Plant Protection Station of Beijing, Beijing 100029, China
  • Online:2009-11-10 Published:2009-11-10

摘要: 应用随机扩增多态性DNA(RAPD)技术,对9个不同地理种群美国白蛾进行了遗传多样性和UPMGA聚类分析。结果表明:25个RAPD引物共扩增191个位点,片段大小在200~2000 bp,其中158个是多态位点,多态百分率为82.72%,遗传距离指数在0.2043~0.5108,遗传相似性系数在0.4892~0.7957;9个不同地理种群的美国白蛾可以分成3类:辽宁省和山东类群、北京和天津类群、美国类群,表明美国白蛾不同地理种群间的遗传分化与地理位置相关。

关键词: Bt水稻, 节肢动物, 功能团, 群落, 化学杀虫剂

Abstract: The genetic diversity and UPMGA clustering of nine Hyphantria cunea geographic populations were analyzed by random amplified polymorphic DNA (RAPD) technique. A total of 191 bands were obtained from 25 effective primers, of which, 158 bands were polymorphic, accounting for 82.72%. The genetic distance index of the populations was 0.2043-0.5108, and the similarity coefficient was 0.4892-0.7957. The nine populations could be divided into three groups, i.e., Liaoning and Shandong group, Beijing and Tianjing group, and American group, suggesting that the genetic differentiation of H. cunea populations was correlated with geographical location.

Key words: Bt transgenic rice, Arthropod, Guild, Community, Chemical insecticide