黄瓜幼苗热胁迫响应基因的分离

生态学杂志

黄瓜幼苗热胁迫响应基因的分离

杨寅桂^1，2;娄群峰¹;庄勇¹;张万萍¹;陈劲枫¹

¹南京农业大学园艺学院作物遗传与种质创新国家重点实验室, 南京 210095；²江西农业大学农学院，南昌 330045

收稿日期:2006-12-09 修回日期:1900-01-01 出版日期:2007-07-10 发布日期:2007-07-10

Isolation of heat response-related gene in cucumber seedlings.

YANG Yin-gui^1,2; LOU Qun-feng¹; ZHUANG Yong¹; ZHANG Wan-ping¹; CHEN Jin-feng¹

¹State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;²College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China

Received:2006-12-09 Revised:1900-01-01 Online:2007-07-10 Published:2007-07-10

摘要/Abstract

摘要： 为了理解黄瓜耐热性或热伤害产生的分子机理，以耐热品种‘西双版纳黄瓜’和热敏品种‘二早子’为供试材料，对幼苗进行38 ℃热激处理2 h。采用cDNA－AFLP技术比较热激前后基因表达差异，分离黄瓜热激响应基因, 并利用RT－PCR技术检测分离基因的表达。结果表明：从‘二早子’中分离到chrr182(cucumber heat response related) 特异片段，经比对发现该片段仅与转录修复偶联因子（transcription repair coupling factor－TRCF）同源；该基因的表达在38 ℃下被诱导增强；黄瓜幼苗耐热性或热伤害的发生可能与TRCF基因的响应有关。

关键词: 沙柳, 灌丛, 植株形态, 气流结构, 防风效应

Abstract: To understand the molecular mechanism of cucumber’s heat tolerance or heat injury, heat-tolerant cultivar ‘Xishuangbanna’ and heat-sentitive culticar‘Erzaozi’ were selected as test materials, and their seedlings were heat treated (38 ℃) for 2 hours. The gene was isolated with cDNA-AFLP technique, and the gene expression was detected by RT-PCR technique. A specific fragment of heat response, chrr182 (cucumber heat response-related), was isolated from the cDNA of ‘Erzaozi’ under heat stress, and the results of blastx in GenBank of NCBI indicated that chrr182 was in homology with transcription-repair coupling factor gene. The expression of chrr182 was enhanced under the inducement at 38 ℃ for 2 hour. The heat tolerance or heat injure of cucumber seedlings could be related to the response of transcription-repair coupling factor gene under heat stress.

Key words: Salix psammophila, Shrub, Plant morphology, Flow structure, Wind-prevention effect

杨寅桂;娄群峰;庄勇;张万萍;陈劲枫. 黄瓜幼苗热胁迫响应基因的分离[J]. 生态学杂志.

YANG Yin-gui^1,2; LOU Qun-feng¹; ZHUANG Yong¹; ZHANG Wan-ping¹; CHEN Jin-feng¹ . Isolation of heat response-related gene in cucumber seedlings.[J]. cje.

[1]	刘美, 陈亚梅, 崔宁洁, 马志良. 模拟增温对高寒灌丛生长季土壤呼吸的影响 [J]. 生态学杂志, 2021, 40(4): 1038-1048.
[2]	王玉洁, 周炳江, 黄郑雯, 孙荣卿, 杨宽, 杨霖, 程希平. 元江干热河谷林地内外潜在蒸散发量的变化及其驱动因素 [J]. 生态学杂志, 2021, 40(2): 501-511.
[3]	张安宁,刘任涛,刘佳楠,赵娟,常海涛,陈蔚. 干旱风沙区柠条枯落物对土壤节肢动物群落的影响 [J]. 生态学杂志, 2020, 39(7): 2383-2391.
[4]	常兆丰,朱淑娟,张剑挥,赵鹏,段晓峰. 民勤绿洲边缘积沙带的稳定性动态 [J]. 生态学杂志, 2020, 39(4): 1300-1308.
[5]	张荣, 董洪君, 周润惠, 余飞燕, 王敏, 陈聪琳, 喻静, 郝建锋. 四川夹金山灌丛群落根系特征对土壤抗冲性的影响 [J]. 生态学杂志, 2020, 39(11): 3558-3566.
[6]	孟莹, 姜鹏, 方缘. 大气水分亏缺对中国两种典型草地生态系统总初级生产力的影响 [J]. 生态学杂志, 2020, 39(11): 3633-3642.
[7]	刘明虎,张建平,苏智,高君亮,赵英铭,马迎宾,满达,陆占东,王志刚. 绿洲防护林缓减冰雹灾害的动力学机制 [J]. 生态学杂志, 2019, 38(9): 2833-2839.
[8]	肖元明,杨路存,聂秀青,李长斌,熊丰,王玲玲,周国英. 坡位对高寒灌丛群落谱系多样性和谱系结构的影响 [J]. 生态学杂志, 2019, 38(6): 1611-1619.
[9]	张喜,霍达,向凯旋,侯贻菊,谢涛,崔迎春. 样地面积对黔中喀斯特石漠灌丛林植物多样性的影响 [J]. 生态学杂志, 2019, 38(5): 1305-1313.
[10]	陈丽茹,李秧秧. 陕北水蚀风蚀交错带沙柳和柠条叶光合作用对降雨改变的响应 [J]. 生态学杂志, 2019, 38(10): 2941-2949.
[11]	李巧燕,来利明,周继华,姜联合,杨柳,易三桂,孙清琳,郑元润. 鄂尔多斯高原草地灌丛化不同阶段主要植物水分利用特征 [J]. 生态学杂志, 2019, 38(1): 89-96.
[12]	王博,刘静,李有芳,张欣,李强,刘嘉伟. 不同损伤条件下沙柳直根力学特性的自修复差异 [J]. 生态学杂志, 2018, 37(12): 3549-3555.
[13]	王鑫1,2,3,4，杨德刚1*，熊黑钢2，柳妍妍1,2,3，公延明1,2,3. 新疆干旱区8种常见灌丛生物量碳和土壤有机碳特征 [J]. 生态学杂志, 2016, 35(8): 1996-2002.
[14]	韩兵兵,王孝安*,李静,王丽娟,原志坚. 黄土高原灌丛对人工油松幼树生长的影响 [J]. 生态学杂志, 2016, 35(12): 3197-3204.
[15]	张旭1,李家湘1,2**,喻勋林1,谢宗强2,寻院3. 湖南大围山杜鹃灌丛木本植物种群空间格局 [J]. 生态学杂志, 2015, 34(11): 3034-3039.