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生态学杂志 ›› 2011, Vol. 30 ›› Issue (07): 1479-1483.

• 研究报告 • 上一篇    下一篇

一株多菌灵高效降解菌的分离鉴定及特性

林秀,侯振安**,赵思峰,谢磊,李会杰,李谟志   

  1. 石河子大学农学院资源与环境科学系, 新疆生产建设兵团绿洲生态农业重点实验室, 新疆石河子 832003
  • 出版日期:2011-07-08 发布日期:2011-07-08

A high efficient carbendazim-degrading bacterial strain: Its isolation and identification.

LIN Xiu, HOU Zhen-an**, ZHAO Si-feng, XIE Lei, LI Hui-jie, LI Mo-zhi   

  1. Key Laboratory of Oasis Eco-Agriculture, Xinjiang Production and Construction, Department of Resources and Environmental Science, Shihezi University, Shihezi 832003, Xinjiang, China
  • Online:2011-07-08 Published:2011-07-08

摘要: 从长期施用多菌灵农药的葡萄园中分离筛选得到一株多菌灵降解菌XJ-H,经Biolog微生物自动分析系统和16S rDNA序列比对以及系统聚类分析等鉴定菌株XJ-H为巴西固氮螺菌(Azospirillum brasilense)。多菌灵降解菌XJ-H可利用多菌灵为唯一碳源、氮源进行生长,将其接种在600 mg·L-1多菌灵的无机盐培养基中,11 d时多菌灵的降解率达到95.6%,平均降解能力为52.2 mg·L-1·d-1

关键词: 城市生态用地, 功能型生态用地, 服务型生态用地, 生态系统服务

Abstract: By the method of continuous enrichment culture, a high efficient carbendazim-degrading bacterial strain XJ-H was isolated from the grape vineyard soil with a long-term application of carbendazim in Shihezi of Xinjiang, Northwest China. Through the Biolog analysis, 16S rDNA sequences comparison, and phylogenetic analysis, the XJ-H was identified as Azospirillum brasilense. The XJ-H could utilize carbendazim as the sole carbon or nitrogen source. After inoculated into the mineral salt medium containing 600 mg·L-1 of carbendazim, the XJ-H could degrade 95.6% of carbendazim within 11 d, with an average degradation rate of 52.24 mg·L-1·d-1.

Key words: Urban ecological land, Ecological land for functioning, Ecological land for service, Ecosystem service