紫茎泽兰水提液的化感潜势及其渗透压的干扰效应

生态学杂志

紫茎泽兰水提液的化感潜势及其渗透压的干扰效应

杨国庆^1,2;万方浩¹;刘万学¹

¹中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京 100193; ²扬州大学园艺与植物保护学院，江苏扬州 225009

收稿日期:2008-05-27 修回日期:1900-01-01 出版日期:2008-12-10 发布日期:2008-12-10

Allelopathic potential of Ageratina adenophora Spengel aqueous extracts and interference effect of their osmotic pressure.

YANG Guo-qing^1,2; WAN Fang-hao¹; LIU Wan-xue¹

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China; ²College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangshu, China

Received:2008-05-27 Revised:1900-01-01 Online:2008-12-10 Published:2008-12-10

摘要/Abstract

摘要： 采用3种方法（整株、剪碎和风干）和处理时间（6、24和48 h），制备了紫茎泽兰地上部分植株不同类型的水提液，分别评价了它们对旱稻种子萌发和幼苗生长的化感潜势，比较分析了不同类型水提液的渗透压值，并检测了该范围内的渗透压对旱稻种子萌发和幼苗生长的影响。结果表明：紫茎泽兰不同类型水提液对旱稻产生了明显的化感抑制潜势，3种处理方法的水提液化感潜势大小依次为风干、剪碎和整株，且这种作用随着水提液处理时间的延长而增强。不同处理方法的水提液渗透压大小也依次为风干、剪碎和整株，并随着处理时间和浓度的增加，水提液渗透压也在增强。对应于不同处理水提液而逐渐增加的渗透压对旱稻种子萌发和幼苗生长产生了逐步增强的抑制作用。表明渗透压是紫茎泽兰水提液化感潜势评估重要的干扰因子，尤其在剪碎或风干植株的处理中，建议采用整株水提法。

关键词: 微卫星DNA, 分离, 富集

Abstract: Different type aqueous extracts from the above-ground part of Ageratina adenophora Sprengel were prepared under the treatments of whole plant, clipped plant and air-dried plant for 6, 24 and 48 hours, aimed to evaluate their allelopathic potential to the seed germination and seedling growth of upland rice (Oryza sativa L.), and examine the interference effect of their osmotic pressure on the allelopathic potential. It was found that these aqueous extracts had significant allelopathic inhibitory potential, with the sequence of the treatment air-dried plant>clipped plant>whole plant, and of the treating time 48 h>24 h>6 h. The osmotic pressure of the aqueous extracts was also in the sequence of the treatment air-dried plant>clipped plant>whole plant, and increased with increasing concentration and treating time. The interference effect of the osmotic pressure was increased with increasing osmotic pressure, suggesting that osmotic pressure could be an important factor which interfered in the evaluation of the allelopathic potential of aqueous extracts from A. adenophora, especially from its air-dried and clipped plants. It was proposed that whole-plant treatment should be preferred in the preparation of A. adenophora aqueous extracts.

Key words: Microsatellite DNA, Isolation, Enrichment

杨国庆;万方浩;刘万学. 紫茎泽兰水提液的化感潜势及其渗透压的干扰效应[J]. 生态学杂志.

YANG Guo-qing^1,2; WAN Fang-hao¹; LIU Wan-xue¹. Allelopathic potential of Ageratina adenophora Spengel aqueous extracts and interference effect of their osmotic pressure.[J]. cje.

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