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南海野生斑节对虾的线粒体16S rRNA基因序列单倍型分析

周发林1,2;江世贵1;姜永杰1;黄建华1   

  1. 1中国水产科学研究院南海水产研究所, 广州 510300;2上海水产大学生命科学与技术学院, 上海 200090
  • 收稿日期:2008-03-26 修回日期:1900-01-01 出版日期:2008-11-10 发布日期:2008-11-10

Analysis of haplotypes of mtDNA 16S rRNA genes of wild tiger prawn (Penaeus monodon) in coastal waters of South China.

ZHOU Fa-lin1,2; JIANG Shi-gui1; JIANG Yong-jie1; HUANG Jian-hua1   

  1. 1South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;2College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China
  • Received:2008-03-26 Revised:1900-01-01 Online:2008-11-10 Published:2008-11-10

摘要: 采用聚合酶链式反应(PCR)技术对我国南海海域5个地点(三亚、深圳、阳江、湛江、北海)的野生斑节对虾100个个体的mtDNA 16S rRNA 序列进行扩增, 扩增产物经纯化后进行测序。用Clustal_X排序软件对所得的100个mtDNA 16S rRNA序列进行比对。通过ARLEQUIN软件对所得100个mtDNA 16S rRNA序列进行比较分析,共检测出28个变异位点,19种单倍型。19单倍型序列的碱基组成显示出较高的A+T比例(69.0%)。19种单倍型序列的遗传距离在0.002~0.033。根据构建的单倍型进化关系网,5个地点群体中的单倍型呈现一种混杂的分布格局。此外,根据单倍型在5个地点群体出现的频率和单倍型进化关系网,单倍型7是最为原始的单倍型,其他18种单倍型均起源于单倍型7。

关键词: 流域, 湿地景观, 破碎化过程

Abstract: A total of 100 Penaeus monodon samples were collected from five locations (Sanya, Shenzhen, Yangjiang, Zhanjiang, and Beihai) in the coastal waters of South China, and PCR technique was used to amplify the mtDNA 16S rRNA of the 100 individuals. The PCR products were purified and sequenced. 440 bp nucleotide sequences of partial 16S rRNA were obtained (some of the marginal sequences were excluded), and 19 haplotypes and 28 variable sites were detected. A high percentage (69.0%) of A+T base composition in the 19 haplotypes was observed. The pairwise distances among the 19 haplotypes ranged 0.002-0.033. The median-joining network of the 19 haplotypes did not distinguish distinctly into five-location groupings of haplotypes. All the haplotypes were scattered throughout the network. Among the 19 haplotypes, the haplotype 7 was ancestral haplotype, and the other 18 haplotypes were originated from haplotype 7, according to the distribution of haplotypes in five locations and median-joining network of 19 haplotypes.

Key words: Watershed, Wetland landscape, Fragmentation process