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生态学杂志 ›› 2011, Vol. 30 ›› Issue (11): 2421-2426.

• 研究报告 • 上一篇    下一篇

承德大窝铺不同海拔与群落类型天然油松种群遗传多样性

张静洁1,李明2,高宝嘉1,3**   

  1. 1河北农业大学林学院, 河北保定 071000;2河北农业大学生命科学学院, 河北保定 071000;3河北北方学院, 河北张家口 075000
  • 出版日期:2011-11-08 发布日期:2011-11-08

Genetic diversity of natural Pinus tabulaeformis populations at different altitudes and in four communities types in Dawopu of Chengde, Hebei Province of North China.

ZHANG Jing-jie1, LI Ming2, GAO Bao-jia1,3**   

  1. 1College of Forestry, Agricultural University of Hebei, Baoding 071000, Hebei, China; 2College of Life Sciences, Agricultural University of Hebei, Baoding 071000, Hebei, China; 3Hebei North University, Zhangjiakou 075000, Hebei, China
  • Online:2011-11-08 Published:2011-11-08

摘要: 利用ISSR技术分析了河北承德大窝铺3个不同海拔、4个不同群落类型的油松天然种群的遗传多样性与遗传分化,探讨了天然油松种群遗传多样性与主要生态因子的关系。结果表明:天然油松种群具有较高的遗传多样性,13个筛选出的随机引物共检测出180个位点,总多态位点比率分别达到73.33%和91.11%。海拔影响油松种群的遗传多样性,中海拔(1070~1130 m)高于高海拔(1230~1350 m)和低海拔(910~970 m)(多态位点比率分别为63.33%、51.67%和59.44%);群落类型与种群的基因组结构变异之间存在一定的内在联系。遗传变异多来自种群内部(分别为90.45%和75.22%),种群分化不明显;群落类型对天然油松种群间遗传变异和基因交流(Gst为0.248,Nm为1.517)的影响高于海拔对其影响(Gst为0.095,Nm为4.738)。可以认为,群落类型对大窝铺天然油松种群遗传分化有较大影响。

关键词: 西洋参, 透光率, 光量子通量密度, 净光合速率

Abstract: To clarify the relationships between natural Pinus tabulaeformis population’s genetic diversity and main ecological factors, this paper analyzed the genetic diversity of different natural P. tabulaeformis populations at three altitudes and in four community types in Dawopu of Chengde, Hebei Province by using ISSR DNA technique. High genetic diversity was observed in these populations. A total of 180 loci were detected with 13 randomly selected primers, and the total percentages of polymorphic loci at altitude and community scales reached 73.33% and 91.11%, respectively. The genetic diversity of the P. tabulaeformis populations was affected by altitude. At medium altitude (1070-1130 m), the genetic diversity was higher than that at high altitude (1230-1350 m) and low altitude (910-970 m), with the percentages of polymorphic loci being 63.33%, 51.67%, and 59.4%, respectively. There existed definite inherent associations between community type and genomic variation. The genetic variation mainly occurred within the populations (90.45% at altitude scale and 75.22% at community scale). As compared with altitude, community type had stronger effects on the genetic variation and gene flow among the P. tabulaeformis populations, with Gst=0.248 and Nm=1.517 under effects of altitude, and Gst=0.095 and Nm=4.738 under effects of community type. It could be concluded that the genetic differentiation of natural P. tabulaeformis populations in Dawopu was more affected by community type.

Key words: Panax quinquefolium, Light transmission rate, Photon flux density, Net photosynthetic rate