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离子强度与水分饱和度对大肠杆菌在多孔介质中迁移和分布的耦合影响

康佳1,2,杨立琼1,石亚楠1,庄杰1*   

  1. 1中国科学院沈阳应用生态研究所, 沈阳 110016;2中国科学院大学, 北京 100049)
  • 出版日期:2020-02-10 发布日期:2020-02-10

Coupling effects of ionic strength and water saturation on transport and distribution of Escherichia coli in porous medium.

KANG Jia1,2, YANG Li-qiong1, SHI Ya-nan1, ZHUANG Jie1*   

  1. (Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; University of Chinese Academy of Sciences, Beijing 100049, China).
  • Online:2020-02-10 Published:2020-02-10

摘要: 离子强度和水分饱和度对微生物在多孔介质中的迁移、滞留等环境行为有重要影响。本试验以自发光大肠杆菌T7为目标微生物,通过柱迁移实验和实时成像技术探究了离子强度(2、50、100 mM)和土壤水分饱和度(85%、100%)对大肠杆菌T7在石英砂中迁移和分布特征的影响。同离子强度下,大肠杆菌T7在饱和(水分饱和度100%)条件下的迁移能力强于非饱和(水分饱和度85%)条件。离子强度100 mM时,大肠杆菌T7在饱和柱中出流百分比Meff为34%,非饱和柱中为8%。一维对流扩散模型拟合结果与迁移实验结果相符,100 mM时非饱和条件下的一级吸附速率katt是饱和条件下的8.7倍。DLVO作用能和毛管力作用能计算结果分析表明,大肠杆菌T7在非饱和条件下迁移能力下降的主要原因是毛管力作用下的气液界面附着。饱和条件下,提高离子强度会促进大肠杆菌T7的滞留。离子强度从2 mM提高至100 mM时,大肠杆菌T7在饱和柱内的滞留百分比Mres从47%增加至58%,其主要原因是双电层静电斥力减小以及大肠杆菌T7平均半径增加。非饱和条件下,提高离子强度也会促进大肠杆菌T7的滞留,且促进程度强于饱和条件。离子强度从2 mM提高至100 mM时,大肠杆菌T7在非饱和柱内的Mres从46%增加至84%,增幅是饱和柱的3.5倍,其主要原因是静电斥力减弱以及毛管力增强。实时成像结果显示,饱和柱中大肠杆菌T7在入口处滞留量较高,提高离子强度会促进入口处滞留。随着时间延长,滞留曲线随深度呈指数递减分布。非饱和条件下,大肠杆菌滞留曲线出现非单调分布,减小离子强度使滞留最大值向出口处移动。

关键词: 生长率, 人工夜间灯光, 功能性状, 香樟, 城市生态系统

Abstract: Ionic strength and water saturation have significant impacts on transport and deposition of bacteria in porous medium. In this study, column breakthrough experiments with IVIS Spectrum System were performed to investigate the effects of ionic strength and water saturation on transport and retention of target bacteria Escherichia coli T7 under three levels of ionic strength (2, 50, 100 mM) and two levels of water saturation (85%, 100%). At same ionic strength, the migration of E. coli T7 under saturated condition was stronger than that under unsaturated condition.Meff of saturated and unsaturated column was 34% and 8% at 100 mM, respectively. In line with above results, the onesite kinetic estimated parameterkatt of unsaturated column was 7.7 times higher than the corresponding values of saturated column at 100 mM. Results of DLVO and capillary force showed that decrease of E. coli T7 migration under unsaturated condition was caused by adsorption of airwater interface due to capillary force. In saturated column, increase of ionic strength promoted deposition of E. coli T7. With ionic strength increasing from 2 mM to 100 mM, Mres of saturated column increased from 47% to 58%, with decrease of electrostatic repulsion and increase of radius of E. coli T7 being the causes. Increase of ionic strength promoted deposition of E. coli T7 under unsaturated condition, which was more significant than that under saturated condition. With ionic strength increasing from 2 mM to 100 mM, Mres of unsaturated columns enhanced from 46% to 84%. Increase of Mres under unsaturated condition was 2.5 times higher than that under saturated condition. The main reasons were decreasing of electrostaticrepulsion and increasing of capillary force. Results of IVIS Spectrum System showed that deposition of E. coli T7 was more significant at inlet of saturated columns, and increasing ionic strength promoted retention. The retention profiles of saturated column were prone to be exponential gradually over time. The retention profiles were non-monotonic in unsaturated column. As ionic strength decreased, the position of max retention quantity moved to outlet of the columns.

Key words: urban ecosystem, functional trait, growth rate., Cinnamomum camphora, artificial nighttime light