欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志 ›› 2020, Vol. 39 ›› Issue (12): 3979-3988.doi: 10.13292/j.1000-4890.202012.023

• 研究报告 • 上一篇    下一篇

光、氮及其互作对设施绿竹叶片碳氮代谢的影响

尹子旭1,卢玉生2,张美曼1,姜小雨1,严欣荣1,黄兰鹰1,官凤英1*   

  1. 1国家林业和草原局/北京市共建竹藤科学与技术重点实验室, 北京 100102;2江西省林业科学院, 南昌 330013)
  • 出版日期:2020-12-10 发布日期:2021-06-10

Interactive effects of light intensity and nitrogen supply on leaf carbon and nitrogen metabolism of Dendrocalamopsis oldhami.

YIN Zi-xu1, LU Yu-sheng2, ZHANG Mei-man1, JIANG Xiao-yu1, YAN Xin-rong1, HUANG Lan-ying1, GUAN Feng-ying1*   

  1. (1Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and Technology, Beijing 100102, China; 2Jiangxi Academy of Forestry, Nanchang 330013, China).
  • Online:2020-12-10 Published:2021-06-10

摘要: 本试验以日光温室南、北两列绿竹为研究对象,南、北两列绿竹冠层光照强度分别为强光(Ls)和弱光(Ln),每列绿竹分别设置不施氮(N0)、低氮(N1: 325 g·丛-1)、高氮(N2: 650 g·丛-1)3个氮肥水平,探究不同光照强度和氮素水平对设施绿竹新叶、老叶碳氮代谢的调控效应,探明设施绿竹生长的适宜光氮条件。结果表明,未施氮时,Ls处理下绿竹新叶叶绿素含量、荧光参数ABS/CSo、φPo显著小于Ln,碳氮代谢相关酶活性、有机碳和全氮含量显著大于Ln,PIABS和C/N无显著差异;氮肥添加后,Ln和Ls处理下新叶叶绿素含量达最大的氮肥水平分别为N1/N2和N2,ABS/CSo最大均为N0,φPoPIABS最大均分别为N2和N2,谷氨酰胺合成酶(GS)、硝酸还原酶(NR)、淀粉酶(AMY)和蔗糖合成酶(SS)活性最大均为N2和N2,有机碳含量最大分别为N1/N2和N1,全氮含量最大分别为N1/N2和N2,C/N最大分别为N0和N0/N1。此外,绿竹叶片叶绿素荧光参数和碳氮代谢相关酶活性对不同氮肥水平响应程度低于不同光强,尤其在老叶中更明显。相比新叶,老叶PIABS在强光下小于弱光,互作效应对其NR、SS酶活性影响未达显著,C/N也在未施氮时达到最小值。因此,本试验中光强因素是绿竹叶片碳氮代谢变化的主导因素,南列绿竹因其较强光照会抑制其叶片功能,短期内可通过自身防御系统保护叶片整体功能,北列绿竹因较弱光照生长速率和吸收氮素能力较慢使叶片对氮素敏感性较差。氮肥添加可增大设施绿竹叶片碳氮代谢活性,但二者并未呈正比,新叶对氮的响应较老叶敏感,高氮处理(N2)使叶片碳氮代谢功能包括叶绿素含量、碳氮代谢相关酶活性、全氮积累量增大,并加快生长速率。

关键词: 光强, 氮肥, 互作效应, 叶绿素, 荧光参数, 碳氮代谢相关酶, 碳氮比

Abstract: To explore the regulatory effect of different light intensity and nitrogen level on carbon-nitrogen metabolism in young and old bamboo leaves, and to seek suitable light and nitrogen conditions for the growth of Dendrocalamopsis oldhami, bamboo culms cultivated in solar greenhouse were treated under conditions of a permutation of two light intensity levels (Ls for strong and Ln for weak) and three nitrogen levels (N0 for no nitrogen, N1 for low nitrogen and N2 for high nitrogen). The results showed that, when nitrogen fertilizer was not applied, the content of chlorophyll and the ABS/CSo, φPo of young leaves under Ls treatment were significantly lower than that of Ln, the activity of enzymes related to carbonnitrogen metabolism, organic carbon and total nitrogen content were significantly higher than that of Ln, and with no difference in PIABS and C/N. Under Ln and Ls treatments with nitrogen supply, the maximum chlorophyll content of young leaves was found with N1/N2 and N2, the maximum ABS/CSo occurred with N0, the maximum φPo orPIABS occurred with N2 and N2, the maximum GS, NR, AMY, SS occurred with N2 and N2, the maximum organic carbon occurred with N1/N2 and N1, the maximum total nitrogen occurred with N1/N2 and N2, and the maximum C/N occurred with N0 and N0/N1, respectively. In addition, the responses of chlorophyll fluorescence parameters and the activities of enzymes related to carbonnitrogen metabolism to different nitrogen fertilization levels were lower than that to different light intensities, especially for old leaves. Compared with young leaves, PIABS of old leaves were lower under Ls than under Ln. The interactive effects of light intensity and nitrogen supply on the enzyme activities of NR and SS of old leaves were not significant, with C/N of old leaves reaching the minimum value under the condition of no nitrogen supply. Our results suggest that light intensity was the dominant factor affecting carbon-nitrogen metabolism of D. oldhami leaves. The southward D. oldhami can inhibit its leaf function due to strong light intensity. In the short term, the overall leaf function can be protected by its own defense system. Because of the weak light on the north row, the growth rate and the ability to absorb nitrogen were low, and the leaf sensitivity to nitrogen was poor. Nitrogen addition increased carbon-nitrogen metabolic activity, but they were not proportional. Young leaves were more sensitive to nitrogen supply than old leaves. High nitrogen level (N2) increased the carbon-nitrogen metabolism of D. oldhami leaves, including chlorophyll content, activity of enzymes related to carbonnitrogen metabolism, total nitrogen accumulation, and thus accelerated growth rate.

Key words: light intensity, nitrogen fertilization, interaction effect, chlorophyll, fluorescence parameter, enzyme related to carbon-nitrogen metabolism, ratio of carbon to nitrogen (C/N).