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生态学杂志 ›› 2020, Vol. 39 ›› Issue (12): 3924-3933.doi: 10.13292/j.1000-4890.202012.022

• 研究报告 • 上一篇    下一篇

不同种源白栎幼苗叶片对干旱胁迫的响应及抗旱性评价

熊仕发1,吴立文1,陈益存1,高暝1,江先桂2,李清源2,黄述河2,汪阳东1*   

  1. 1中国林业科学研究院亚热带林业研究所, 杭州 311400; 2福建省清流国有林场, 福建清流 365300)
  • 出版日期:2020-12-10 发布日期:2021-06-10

Response of leaf of Quercus fabri seedlings from different provenances to drought stress and drought resistance evaluation.

XIONG Shi-fa1, WU Li-wen1, CHEN Yi-cun1, GAO Ming1, JIANG Xian-gui2, LI Qing-yuan2, HUANG Shu-he2, WANG Yang-dong1*#br#   

  1. (1Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China; 2Fujian State-owned Qingliu Forest Farm, Qingliu 365300, Fujian, China).
  • Online:2020-12-10 Published:2021-06-10

摘要: 为了解不同种源白栎苗期抗旱性差异,以期为白栎栽培及耐旱品种选育提供依据,采用PEG-6000溶液模拟干旱环境,测定不同程度干旱胁迫对8个不同种源白栎生理生化指标的影响,并通过主成分分析和隶属函数法综合评价8个种源白栎的抗旱性强弱。结果表明:随着干旱胁迫程度加深,不同种源白栎幼苗叶片的相对含水量和水势均呈下降趋势;MDA含量呈显著增长趋势,而SOD、POD和CAT活性均呈现先升高后降低趋势;可溶性糖、蛋白质和游离脯氨酸含量均呈现先升高再降低的趋势,而甜菜碱含量则呈现持续升高趋势;利用主成分分析和隶属函数对10个抗旱性指标进行综合分析,得出8个白栎种源抗旱性强弱顺序为:福建武夷山>贵州黄平>江西宜春>广西全州>湖北利川>湖北黄石>广西柳州>浙江西湖。

关键词: 干旱胁迫, 白栎, 生理生化, 抗性评价, 隶属函数

Abstract: To explore the differences in drought resistance of Quercus fabri seedlings from different provenances and thus provide a basis for Q. fabri cultivation and the breeding of drought-tolerant varieties, PEG-6000 solution was used to simulate drought stress. The effects of different degrees of drought stress on the physiological and biochemical indices of Q. fabri were measured. Drought resistance of Q. fabrifrom eight provenances was comprehensively evaluated through principal component analysis and membership function method. The results showed that, with increasing drought stress, the relative water content and water potential of Q. fabrileaves from different provenances all exhibited a downward trend. MDA content showed a significant increase trend. The activities of SOD, POD and CAT all showed a trend of first increasing and then decreasing. The contents of soluble sugar, protein, and free proline all showed a trend of first increasing and then decreasing, while the content of betaine showed an increasing trend. Theresults of principal component analysis and membership function with 10 drought resistance indices being considered showed that the drought resistance of eight provenances ofQ. fabri was ranked as follows: Fujian Wuyishan>Guizhou Huangping>Jiangxi Yichun>Guangxi Quanzhou>Hubei Lichuan>Hubei Huangshi>Guangxi Liuzhou>Zhejiang West Lake.

Key words: drought stress, Quercus fabri, physiological biochemistry, resistance assessment, membership function.