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• 研究报告 • 上一篇    下一篇

稀土元素铈对斑马鱼肝脏的遗传毒性

贺彦斌1,2,台培东1,孙梨宗1,2*,郭橙2,3,刘志红4,丛仕龙3   

  1. 1中国科学院污染生态与环境工程重点实验室, 中国科学院沈阳应用生态研究所, 沈阳 110016;2中国科学院大学, 北京 100049;3辽宁石油化工大学, 辽宁抚顺 113001; 4Deakin University, Victoria 001138, Australia)
  • 出版日期:2018-09-10 发布日期:2018-09-10

Genotoxicity of rare-earth element cerium on zebrafish (Danio rerio) livers.

HE Yan-bin1,2, TAI Pei-dong1, SUN Li-zong1,2*, GUO Cheng2,3, LIU Zhi-hong4, CONG Shi-long3   

  1. (1Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 2University of Chinese Academy of Sciences, Beijing 100049, China; 3Liaoning Shihua University, Fushun 113001, Liaoning, China; 4Deakin University, Victoria 001138, Australia).
  • Online:2018-09-10 Published:2018-09-10

摘要: 以稀土元素中丰度最大的铈(Ce)为代表污染物,以斑马鱼(Danio rerio)为受试生物,采用RAPD-PCR和MSAP-PCR技术研究Ce对斑马鱼基因组DNA损伤及DNA甲基化的影响。结果表明,不同浓度Ce3+(0、5、10和20 μmol·L-1)胁迫斑马鱼28天后,Ce3+主要在斑马鱼肝脏中富集(BCF:5.49~9.33),其次为腮(BCF:3.58~4.49)和肌肉(BCF:0.13~0.25);RAPD-PCR分析显示,Ce3+(>10 μmol·L-1)胁迫能够诱导斑马鱼肝脏DNA的损伤;MSAP-PCR分析显示,Ce3+胁迫引起的斑马鱼肝脏基因组DNA甲基化总变化率分别为8.93%(CK)、9.12%(5 μmol·L-1)、15.56%(10 μmol·L-1)和28.83%(20 μmol·L-1),其中低浓度胁迫下,斑马鱼肝脏基因组DNA去甲基化(D)型显著增加,高浓度下,甲基化(M)型显著增加,DNA甲基化多态性亦随胁迫浓度的增大而变大;测序分析结果显示,这些特异性甲基化条带与zinc finger protein STZ/ZAT10、ABC transporter 1、cell cycle control phosphatase superfamily protein等基因具有较高的同源性;与RAPD标记相比,DNA甲基化标记对Ce3+的胁迫响应具有高敏感性。研究结果深化了对稀土元素生物学效应的认识。

关键词: 播期, 播量, 氮素运转, 水分消耗, 旱地小麦

Abstract: Cerium (Ce) is the most abundant rare-earth element. Using RAPD-PCR (random amplified polymorphic DNA-PCR) and MSAP-PCR (methylation-sensitive amplified polymorphism-PCR), the effects of Ce on genomic DNA damage and DNA methylation in zebrafish (Daniorerio) were examined. The results showed that, after the fish was exposed to different concentrations of Ce3+ (0, 5, 10 and 20 μmol·L-1) for 28 days, Ce3+ was mainly enriched in the liver (bioconcentration factors BCF: 5.49-9.33), followed by gill (BCF: 3.58-4.49) and muscle (BCF: 0.13-0.25). The results of RAPD-PCR showed that Ce3+ (>10 μmol·L-1) could induce DNA damage in zebrafish livers. The results of MSAP-PCR showed that the change rates of total genomic DNA methylation of zebrafish livers caused by Ce3+ stress were 8.93% (CK), 9.12% (5 μmol·L-1), 15.56% (10 μmol·L-1), and 28.83% (20 μmol·L-1), respectively. The demethylation (D) of genomic DNA in zebrafish livers significantly increased under the low concentration of Ce3+, while the methylation (M) significantly increased under the high concentration of Ce3+. Meanwhile, the methylation polymorphism increased with the increases of Ce3+ concentration. Results from sequencing analysis showed that these specific bands had high homo logy with the genes of zinc finger protein STZ/ZAT10, ABC transporter 1, and cell cycle control phosphatase superfamily protein. Compared to RAPD markers, DNA methylation markers were highly sensitive to Ce3+ stress. These results contribute to our understanding of the biological effects of rare earth elements.

Key words: nitrogen translocation, dryland wheat, water consumption, seeding rate, sowing date