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温度胁迫对日本鳗草(Zostera japonica)叶际可培养细菌的影响

韩秋影1, 2,张泽玉3,刘红霞4,刘伟妍5,张晓黎2*#br#   

  1. (1海南热带海洋学院, 海南三亚 572022; 2中国科学院烟台海岸带研究所, 中国科学院海岸带环境过程与生态修复重点实验室, 山东烟台 264003; 3威高药业股份有限公司, 山东威海 264200; 4山东吉威医疗制品有限公司, 山东威海 264200; 5吉林农业大学生命科学学院, 长春 130118)
  • 出版日期:2017-09-10 发布日期:2017-09-10

Effects of rising temperature on phyllosphere culturable bacteria of Zostera japonica.

HAN Qiu-ying1,2, ZHANG Ze-yu3, LIU Hong-xia4, LIU Wei-yan5, ZHANG Xiao-li2*#br#   

  1. (1Hainan Tropical Ocean University, Sanya 572022, Hainan, China; 2Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Chinese Academy of Sciences, Yantai 264003, Shandong, China; 3Weigao Pharmaceutical Co., Ltd., Weihai 264200, Shandong, China; 4Shandong Geoway Medical Products Co., Ltd., Weihai 264200, Shandong, China; 5College of Life Sciences, Jilin Agricultural University, Changchun 130118, China).
  • Online:2017-09-10 Published:2017-09-10

摘要: 叶际微生物对海草的生理代谢和生态功能具有重要意义,目前全球温度升高对日本鳗草叶际微生物的影响还不明确。本研究在实验室条件下通过梯度升温(26、28、30和32 ℃)胁迫实验,利用培养分离、RFLP分型、测序和系统发育分析等手段,研究了温度升高对日本鳗草叶际可培养细菌的影响。随着温度的升高,叶际可培养细菌的数量先增加后降低,32 ℃数量最多。共分类获得363株菌株,分为24个OTU,均来自α和γ变形菌纲,未发现所有温度梯度共有的OTU。海单胞菌(Marinomonas)为最优势的类群,其在28 ℃相对丰度最高,达到86.8%;假单胞菌(Pseudomonas)和Thalassospira属细菌随温度升高锐减;海杆菌属(Marinobacter)和弧菌(Vibrio)在30 ℃被富集;而32 ℃时,红螺菌科(Rhodobacteraceae)和玫瑰杆菌(Ruegeria)激增。26和32 ℃细菌的多样性和均匀度较高,28 ℃最低。

关键词: 植被化学计量特征, 物种多样性, 沙柳群落, 宁夏哈巴湖国家级自然保护区, 油蒿群落

Abstract: Phyllosphere microorganisms play an important role in physiological metabolism and ecological functions of seagrasses. Little is known about the effects of rising temperature on phyllosphere culturable bacteria of Zostera japonica. The abundance, composition, diversity and distribution of phyllosphere culturable bacteria under 26, 28, 30 and 32 ℃ were studied by isolation, counting, RFLP, sequencing and phylogenetic analysis. The abundance of bacteria under different temperatures showed a pattern of 32 ℃>28 ℃>26 ℃>30 ℃. A total of 363 strains were isolated and divided into 24 OTUs. No common OTU was found across all temperatures. All strains derived from α and γproteobacteria, and Marinomonas was the most dominant phyla, which accounted for 86.8% of total abundance under 28 ℃. Pseudomonas and Thalassospira sharply decreased with rising temperature; Marinobacter and Vibrio were enriched under 30 ℃, while Rhodobacteraceae and Ruegeria sharply increased under 32 ℃. The diversity and evenness of phyllosphere culturable bacteria were much higher under 26 and 32 ℃, while the lowest under 28 ℃.

Key words: Salix psammophila community, the Ningxia Habahu National Nature Reserve., Artemisia ordosica community, vegetation stoichiome-try, species diversity