Abstract: The ecological floating bed was enhanced by aerobic denitrifiers and aeration, which improved the removal of nitrogen and organic matter from eutrophic water. The results showed that 54.5% of NH₄⁺-N, 100% of NO₃^--N, 59.8% of TN and 56.3% of COD_Cr were removed by the aerobic denitrifiers enhanced ecological floating bed in seven days at COD_Cr/TN=1.7-2.9 inflow, and the removal efficiencies of TN and COD_Cr were 47.1% and 36.3% higher than in the traditional floating bed, respectively. Additionally, the aerobic denitrifiers enhanced ecological floating bed removed 55.4% of NH₄⁺-N, 100% of NO₃^--N, 48.9% of TN and 65.6% of COD_Cr in seven days at COD_Cr/TN=3.3-5.0 inflow, and the removal efficiencies of TN and COD_Cr were 21.0% and 20.0% higher than in the traditional floating bed, respectively. In the aerobic denitrifiers enhanced ecological floating bed, aeration benefited the removal of organic matter and aerobic denitrifiers would enhance the denitrification under the high level of DO, avoiding the poor performance of the NO₃^--N removal by the aerated ecological floating bed. Monod kinetic model was used to predict the initial removal of TN and COD_Cr by the aerobic denitrifiers enhanced ecological floating bed, with correlation coefficients of about 0.9, suggesting that this model had a good prediction on the initial removal of nitrogen and organic matter.