Abstract: Ecological function zoning comprehensively reflects the different characteristics of regional ecological function, which is an important basis of differentiated spatial governance to natural resource development and ecoenvironmental protection. Aiming at the existent problems of the multiple analytical framework and subjective and complicated weighted value in previous ecological function zoning, the ecological function zoning of Dali Bai Autonomous Prefecture was carried out based on systematic analysis framework of elementstructurefunction. An index system was constructed from three aspects of basal element, interference structure, and dominant services. By the application of SOFM, ecological service zoning, basal element zoning, interference structure zoning, and comprehensive ecological function zoning were conducted. Finally, Dali Bai Autonomous Prefecture was divided into 4 ecological function zones and 10 ecological functional subzones. Through comparing the different zones, it was found that the comprehensive ecological function zoning was mainly controlled by the zoning of primary ecosystem services, with an overlapping rate of 79.98%. By adopting the framework of element, structure, and function, this study systematically described the characteristics and spatial difference of regional ecosystem structure and function, providing a new approach for the zoning of ecological function.