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N添加对油松幼苗土壤酶活性和微生物生物量的影响

吕凤莲1,薛萐2,3,王国梁2,3*,景航2   

  1. 1 西北农林科技大学资源与环境学院, 陕西杨凌 712100; 2西北农林科技大学水土保持研究所, 陕西杨凌 712100; 3中国科学院水土保持与生态环境研究中心, 陕西杨凌 712100)
  • 出版日期:2016-02-10 发布日期:2016-02-10

Effects of N addition on soil enzyme activities and microbial biomass beneath Pinus tabuliformis seedlings.

LU Feng-lian1, XUE Sha2,3, WANG Guo-liang2,3*, JING Hang2   

  1. (1College of Natural Resources and Environment, Northwest A&F University, Yangling, 712100, Shaanxi, China; 2 Institute of Soil and Water Conservation, Northwest A&F University, Yangling 712100, Shaanxi, China; 3Institute of Soil and Water Conservation, CAS & MWR, Yangling 712100, Shaanxi, China)
  • Online:2016-02-10 Published:2016-02-10

摘要: 通过连续6年对盆栽油松(Pinus tabuliformis)幼苗进行不同水平N添加(0、2.8、5.6、11.2、22.4和44.8 g N·m-2·a-1),研究了N添加对土壤酶活性(蔗糖酶、脲酶和碱性磷酸酶)和微生物生物量碳、氮的影响。结果表明:N添加水平低于11.2 g N·m-2·a-1时,对油松幼苗土壤蔗糖酶和脲酶活性均有促进作用,但过量N添加,尤其超过22.4 g N·m-2·a-1时,蔗糖酶、脲酶和碱性磷酸酶活性均不再提高,甚至表现为抑制作用;N添加为5.6 g N·m-2·a-1,微生物生物量碳显著增加,微生物生物量氮无显著变化,而当N添加达到11.2 g N·m-2·a-1时,微生物生物量氮呈显著增加的趋势;土壤蔗糖酶、脲酶活性与微生物生物量碳和微生物生物量氮间均有显著相关关系,而3种酶活性间、微生物生物量碳、氮间均无显著相关性;土壤酶活性和微生物生物量与地上生物量、土壤有机碳和全氮含量显著相关。油松在5.6~11.2 g N·m-2·a-1水平下生长达到最佳。目前黄土高原地区N沉降水平(2.06 g N·m-2·a-1)有利于该地区油松林生长;可以通过适当增施N肥的方式提高油松生长。

关键词: 导数光谱, 高光谱遥感数据, 大豆, 高清数码影像, 氮平衡指数, 植被指数, 无人机

Abstract: With a pot experiment, we compared the effects of N addition (0, 2.8, 5.6, 11.2, 22.4 and 44.8 g N·m-2·a-1) on soil enzyme activities (invertase, urease and alkaline phosphatase) and microbial biomass beneath Pinus tabuliformis seedlings after 6 years consecutive treatment. The results showed that soil invertase and urease activities were improved when the N addition was below 11.2 g N·m-2·a-1, while soil invertase, urease and alkaline phosphatase activities did not increase, even declined with a higher rate of N addition, especially more than 22.4 g N·m-2·a-1. The microbial biomass C significantly increased at N addition ≥5.6 g N·m-2·a-1, while microbial biomass N significantly increased at N addition ≥11.2 g N·m-2·a-1, compared with the control. Significant correlations were found between soil invertase and urease activities and microbial biomass C and microbial biomass N. No significant correlations, however, were found among the three enzyme activities, or between the microbial biomass C and N. Soil enzyme activities and microbial biomass were significantly correlated with aboveground biomass, soil organic carbon and total nitrogen content. Additionally, the growth of P. tabuliformis seedlings reached the maximum at the N addition levels of 5.6-11.2 g N·m-2·a-1. The current N deposition level of 2.06 g N·m-2·a-1 on the Loess Plateau can be favorable to P. tabuliformis growth, and the growth of P. tabuliformis would be improved through appropriately enhanced N fertilization.

Key words: soybean., derivative spectrum, unmanned aerial vehicle, high definition digital image, nitrogen balance index, hyperspectral data, vegetation index