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淹水对灰化苔草幼苗生长及抗氧化物酶活性的影响

曹昀1*,郑祥2,杨阳3,陈冰祥1,国志昌1,吴海英1#br#   

  1. (1江西师范大学地理与环境学院, 南昌 330022; 2江西师范大学生命科学学院, 南昌 330022; 3四川省广元市实验中学, 四川广元 628000)〖HT5SS〗
  • 出版日期:2016-12-10 发布日期:2016-12-10

Effects of waterlogging on the growth and antioxidant enzyme activity of Carex cinerascens seedlings.

CAO Yun1*, ZHENG Xiang2, YANG Yang3, CHEN Bing-xiang1, GUO Zhi-chang1, WU Hai-ying1#br#   

  1. (1 School of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China; 2School of Life Sciences, Jiangxi Normal University, Nanchang 330022, China; 3Sichuan Guangyuan Experimental Middle School, Guangyuan 628000, Sichuan, China).
  • Online:2016-12-10 Published:2016-12-10

摘要: 淹水是影响湿地植物生长繁殖及种群分布的重要因素。为明确淹水对植物生长及生理特性的影响,本研究以灰化苔草(Carex cinerascens)幼苗为研究对象,设对照(常规浇水)、全淹水、1/2淹水和1/4淹水4种处理,测定其在不同淹水条件的形态指标(株高、萌发数)和生理指标(SPAD、MDA、SOD、POD和CAT)的变化。结果表明:淹水胁迫会抑制灰化苔草幼苗生长,表现为株高降低,叶缘发黄;淹水对灰化苔草根茎的萌发也有影响,适度的淹水有利于其萌发;淹水导致灰化苔草幼苗叶片的相对叶绿素含量降低,抑制其光合作用;淹水胁迫加剧灰化苔草叶片的膜脂过氧化程度,促使丙二醛含量增高,对应的抗氧化物酶POD、CAT和SOD含量升高,说明淹水胁迫会破坏植物体内活性氧的产生与清除系统之间的动态平衡;淹水胁迫将加剧膜脂过氧化作用,抑制灰化苔草的正常生长,同时又会加速产生抗氧化物酶来抵抗淹水胁迫带来的不利影响。

关键词: 半冬性小麦, 春性小麦, 氮肥, 氮积累运转, 氮肥利用率

Abstract: Waterlogging is an important factor influencing wetland plant growth, reproduction and population distribution. To clarify the influence of waterlogging on plant growth and physiological characteristics, we set up four treatments: normal watering (control), completely submerged group, 1/2 immersed group and 1/4 immersed group with Carex cinerascens seedlings as the research object, and determined the morphological indicators (plant height, germination) and physiological indicators (SPAD, MDA, SOD, POD and CAT) under different flooding conditions. The results showed that waterlogging stress inhibited the growth of C. cinerascens seedlings, decreased plant height, and the edges of leaves were damaged and yellow. Waterlogging also affected rhizomatic germination of C. cinerascens, and moderate waterlogging would improve its germination. Waterlogging decreased the leaf relative chlorophyll content, inhibiting the photosynthesis of plant leaves. Waterlogging stress aggravated leaf membrane lipid peroxidation, prompted the MDA levels and increased the activities of corresponding antioxidant enzymes POD, CAT and SOD, indicating that the dynamic balance between the scavenging system and the generation of reactive oxygen species was destroyed. Our study suggests that waterlogging stress would aggravate the membrane lipid peroxidation, inhibit the normal growth of C. cinerascens seedlings, and accelerate the production of antioxidant enzymes to counter the adverse effects of waterlogging stress at the same time.

Key words: nitrogen utilization efficiency., springness wheat, nitrogen accumulation and translocation, nitrogen fertilizer, semi-winterness wheat