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油菜素内酯对亚适宜温光盐环境下黄瓜幼苗根系生长及其AsA-GSH循环的影响

吴秀,陆晓民**   

  1. (安徽科技学院, 安徽凤阳 233100)
  • 出版日期:2015-08-10 发布日期:2015-08-10

Effects of brassinolide on the growth and ascorbate-glutathione cycle of cucumber seedling roots under suboptimal temperature, light and salt environment.

WU Xiu, LU Xiao-min**   

  1. (Anhui Science and Technology University, Fengyang 233100, Anhui, China)
  • Online:2015-08-10 Published:2015-08-10

摘要: 以‘津春2号’黄瓜幼苗为试材,研究了24表油菜素内酯(EBR)对亚适宜温光盐环境下黄瓜幼苗根系生长及其抗坏血酸谷胱甘肽(AsAGSH)循环系统的影响。结果表明:亚适宜温光盐环境导致黄瓜幼苗根系的超氧阴离子(O2-·)产生速率及过氧化氢(H2O2)、丙二醛(MDA)含量增加,膜脂过氧化程度加剧,幼苗根系生长显著受抑;EBR能通过提高亚适宜温光盐环境下黄瓜幼苗根系的抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)、脱氢抗坏血酸还原酶(DHAR)等抗氧化酶比活性、抗氧化剂(AsA、GSH)含量以及还原型谷胱甘肽与氧化型谷胱甘肽含量比值(GSH/GSSH)和还原型抗坏血酸与氧化型抗坏血酸比值(AsA/DHA),促进亚适宜温光盐环境下黄瓜幼苗根系体内AsA-GSH循环运转,维持较强的抗氧化性能,显著降低了O2-·产生速率及H2O2、MDA含量,减少膜脂过氧化程度,从而促进了亚适宜温光盐环境下黄瓜幼苗根系的生长。

关键词: 遮荫, 耐冬山茶, 干旱, 气体交换

Abstract: The effects of 24epibrassinolide (EBR) on the growth and ascorbateglutathione (AsA-GSH) cycle in cucumber (‘Jinchun 2’) seedling roots under suboptimal temperature, light and salt environment were studied. The results showed that under the suboptimal environment, the superoxide anion (O2-·) production rate, hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents were increased, and lipid peroxidation was aggravated in cucumber seedling roots, which significantly inhibited the growth of seedling roots. EBR improved the activities of antioxidant enzymes such as ascorbate peroxidase (APX), glutathione reductase (GR) and dehydroascorbate reductase (DHAR), increased the contents of ascorbic acid (AsA) and glutathione (GSH) and the ratios of AsA/DHA and GSH/GSSG in roots of cucumber seedlings under the suboptimal environment. Moreover, EBR effectively promoted the metabolism of AsAGSH cycle in cucumber seedling roots, and maintained a strong antioxidant ability, which significantly reduced the H2O2 and MDA contents and the superoxide anion production rate, reduced the membrane lipid peroxide level, consequently promoting the root growth of cucumber seedlings under the suboptimal temperature, light and salt environment.

Key words: gas exchange., drought, Camellia japonica (Naidong), shading