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河西走廊酒泉地区盐碱土壤中可培养放线菌多样性

李海云1,胡磊1,2,牛世全1**,孔维宝1,达文燕1,韩彩虹1,阎薇如1,耿晖1   

  1. (1西北师范大学生命科学学院, 兰州 730070; 2湖北省农业科学院植保土肥研究所, 武汉 430070)
  • 出版日期:2015-02-07 发布日期:2015-02-07

Diversity of culturable actinomycetes in saline-alkali soil in Jiuquan region of Hexi Corridor.

LI Hai-yun1, HU Lei1,2, NIU Shi-quan1**, KONG Wei-bao1, DA Wen-yan1, HAN Cai-hong1, YAN Wei-ru1, GENG Hui1   

  1. (1College of Life Science, Northwest Normal University, Lanzhou 730070, China; 2Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Science, Wuhan 430070, China)
  • Online:2015-02-07 Published:2015-02-07

摘要:

为了探究甘肃河西走廊酒泉地区盐碱土壤中可培养放线菌种群多样性信息,采用稀释平板涂布法对采集到的4个盐碱土样进行分离培养纯化,并基于16S rRNA基因序列对分离的放线菌株进行系统发育分析。结果显示:根据菌落的表型差异从中共分离到63株放线菌菌株,它们分属于7个亚目、11个科、24个属;其中Streptomyces属为河西走廊酒泉地区盐碱土壤中可培养放线菌的优势菌群,共分离到24株,占放线菌种群总数的38.1%,并且有22株放线菌与已知菌序列的相似性低于98%,占放线菌总数量的34.9%。表明,河西走廊酒泉地区盐碱土壤中的放线菌资源丰富,类群具有多样性。
 

关键词: 小枝长度, 出叶强度, 小枝直径, 叶片数量, 叶面积

Abstract: In order to reveal the population diversity of culturable actinomycetes in saline-alkali soil in Jiuquan region of Hexi Corridor, Gansu, four soil samples were analyzed using dilution plate coating method, and phylogenetic analysis was conducted based on 16S rRNA gene sequencing. The results showed that total 63 strains of culturable actinomycetes were isolated according to their phenotypes, belonging to 24 genera, 11 families and 7 suborders, among which, 24 strains belonging to genus Streptomyces were the dominant populations, accounting for 38.1% of total number of actinomycetes strains. In addition, there were 22 strains of actinomyces with sequence similarity less than 98% compared with the known strains in GenBank, accounting for 34.9% of total number of actinomycetes strains. These results indicate that actinomycetes resources are abundant with high diversity in the saline-alkali soil in Jiuquan region of Hexi Corridor.

Key words: twig length, leafing intensity, leaf number, twig diameter, leaf area