欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志

• 研究报告 • 上一篇    下一篇

茜素络合物对唐鱼耳石标记效果以及生长和存活率的影响

王正鲲,赵天,林小涛**,许忠能,孙军,刘明中   

  1. (暨南大学水生生物研究所, 广东省高校水体富营养化与赤潮防治重点实验室, 广州 510632)
  • 出版日期:2015-01-10 发布日期:2015-01-10

Effects of alizarin complexone (ALC) on otolith marking, growth, and survival of Tanichthys albonubes.

WANG Zheng-kun, ZHAO Tian, LIN Xiao-tao**, XU Zhong-neng, SUN Jun, LIU Ming-zhong   

  1. (Institute of Hydrobiology, Jinan University, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institutes, Guangzhou 510632, China)
  • Online:2015-01-10 Published:2015-01-10

摘要:

用茜素络合物(ALC)对唐鱼(Tanichthys albonubes)进行荧光标记,通过测定唐鱼耳石的标记率、死亡率、SGR和DWG等指标,讨论不同浓度ALC对唐鱼耳石标记效果和对其生长和存活的影响,探讨应用该标记技术追踪唐鱼野外种群迁移行为的可行性。结果表明:在温度28~30 ℃,浓度50 mg·L-1、80 mg·L-1茜素络合物溶液中浸泡24 h,唐鱼仔鱼、稚鱼死亡率为0,其中80 mg·L-1浓度处理组耳石荧光标记环检出率为100%,而在100 mg·L-1浓度下,仔、稚鱼死亡率均达到80%;幼鱼在浓度为80、100 mg·L-1条件下无死亡,其中100 mg·L-1浓度处理组耳石标记环检出率为100%,而150 mg·L-1浓度下幼鱼死亡率为44%;成鱼在100、150 mg·L-1浓度下无死亡,且耳石标记率均为100%,而在200 mg·L-1浓度下成鱼死亡率达到100%;此外,稚鱼在80 mg·L-1最适浓度下浸泡24 h后继续饲养90 d,标记组和对照组死亡率均为0,两组体质量、SGR和DWG差异不显著;唐鱼仔稚鱼、幼鱼和成鱼耳石标记的最适茜素络合物浓度分别为80、100和150 mg·L-1
 

 

关键词: 土壤有机碳, 植被类型, 细根, 凋落物, 土壤全氮

Abstract:

The effects of alizarin complexone (ALC) on otolith marking, growth and survival of Tanichthys albonubes were studied, aiming to improve the applicability of this marking technique in tracing T. albonubes for a better understanding of its movement and migration pattern. Larvae, juveniles with the body length of 5.8-10.5 mm (J5s), juveniles with the body length of 10.8-13.2 mm (J10s), and adults of T. albonubes were selected in the experiment, the water temperature was 28-30 ℃, and immerse time was 24 h. Larvae and J5s of T. albonubes exposed to 50 mg·L-1 and 80 mg·L-1 of ALC had a 100% survival rate, but those exposed to 100 mg·L-1 had a mortality rate of 80%. Under the condition of 80 mg·L-1 ALC, growth rings in otoliths detected by fluorescence microscope (GRFM) were found in all the larvae and J5s. Under 80 mg·L-1 and 100 mg·L-1 of ALC, all J10s survived, but the mortality rate of J10s exposed to 150 mg·L-1 of ALC was 44%. GRFM was found in all J10s under 100 mg·L-1 of ALC. Under 100 mg·L-1 and 150 mg·L-1 of ALC, all adult fish survived with GRFM, but under 200 mg·L-1 of ALC all adult fish died. Cultured for 90 days after exposure to ALC, J5s had the similar body weight, SGR, DWG, and 100% survival rate as compared to the controls (P>0.05). It was suggested that the suitable concentrations of ALC treating larvae, J5s, J10s, and adults of T. albonubes for GRFM were 80 mg·L-1, 100 mg·L-1 and 150 mg·L-1, respectively.
 

Key words: litter, total soil nitrogen, vegetation types, soil organic carbon, fine root