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不同浓度O3对黄檗幼苗叶片生理特征的影响

付伟1,2,邓莉兰1,徐胜2**,何兴元2,陈玮2,李梅2,3,赵诣2,苏丽丽2   

  1. (1西南林业大学园林学院, 昆明 650224; 2森林与土壤生态国家重点实验室, 中国科学院沈阳应用生态研究所, 沈阳 110016; 3辽宁师范大学生命与科学学院, 辽宁大连 116033)
  • 出版日期:2014-09-10 发布日期:2014-09-10

Effects of different O3 concentrations on leaf physiology of Phellodendron amurense Rupr. seedlings.

FU Wei1,2, DENG Li-lan1, XU Sheng2**, HE Xing-yuan2, CHEN Wei2, LI Mei2,3, ZHAO Yi2, SU Li-li2   

  1. (1Faculty of Landscape Architecture, Southwest Forestry University, Kunming 650224, China; 2State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 3Faculty of Life Science, Liaoning Normal University, Dalian 116033, Liaoning, China)
  • Online:2014-09-10 Published:2014-09-10

摘要:

以4年生黄檗(Phellodendron amurense Rupr.)幼苗为实验材料,利用开顶箱模拟法,开展了4个不同浓度O3 40 (对照)、80、120和200 nmol·mol-1的气体熏蒸实验,测定并分析了黄檗叶片光合色素含量、净光合速率、膜脂质过氧化产物和抗氧化酶活性等生理指标的变化规律。结果表明:与CK相比,随着O3浓度增加和处理时间延长,黄檗幼苗叶片叶绿素总含量、类胡萝卜素含量和净光合速率显著下降(P<0.05),最大降幅分别为52.76%、44.57%和54.67%;叶片离子渗漏率、丙二醛含量显著增加(P<0.05),最大增幅分别为101.49%和70.21%。当O3浓度为80 nmol·mol-1时,叶片最大光化学效率(Fv/Fm)无显著变化,而O3浓度为120和200 nmol·mol-1时,Fv/Fm显著下降(P<0.05),表明黄檗幼苗的光合系统功能在超高浓度臭氧下受到了显著伤害。叶片抗氧化超氧化物歧化酶活性在开始处理后的前14 d升高,随后下降,最大降幅为17.22%。O3浓度为120和200 nmol·mol-1时,超氧阴离子(O2-·)含量显著升高(P<0.05);O3浓度为80 nmol·mol-1时,O2-·含量在处理7 d与CK相比显著增加,随后无显著差异。表明黄檗幼苗在生长初期对中等高浓度(80 nmol·mol-1)臭氧熏蒸具有一定的适应和耐受性是因其具有较强抗氧化保护能力,更高浓度的O3或更长时间的熏蒸对叶片光合系统产生破坏。
 
 
 

关键词: 绿色发展, 江西省, 区划, 指标体系

Abstract: Fouryearold Phellodendron amurense Rupr. seedlings were exposed to four levels (40 (CK), 80, 120, and 200 nmol·mol-1) of O3 in opentop champers (OTCs). The changes in photosynthetic pigment contents, net photosynthesis rate, lipid peroxidation and the activity of antioxidative enzymes were periodically measured in leaves of P. amurense. The results showed that with the increasing O3 concentration and exposure time, total chlorophyll (Chl) contents, carotenoid (Car) contents and net photosynthesis rate (Pn) were significantly decreased by 52.76%, 44.57% and 54.67%, respectively (P<0.05); electrolyte leakage and malondialdehyde (MDA) contents were significantly increased by 101.49% and 70.21%, respectively (P<0.05). When the O3 concentration was 80 nmol·mol-1, the maximum photochemical efficiency (Fv/Fm) had no significant change, while a significant decrease in Fv/Fm was observed when the O3 concentration was increased to 120 or 200 nmol·mol-1, showing a significant harm of photosynthetic system function by high O3 concentrations. Superoxide dismutase (SOD) activity increased at the first fourteen days exposure and then decreased by 17.22%. Compared with CK, the superoxideanion (O2-·) content increased significantly (P<0.05) when the O3 concentration was 120 or 200 nmol·mol-1, while only increased significantly in the seventh day  when the O3 concentration was 80 nmol·mol-1. It is concluded that P. amurense can tolerate moderately high concentrations of O3 for a period which is related to its antioxidative ability. Higher O3 concentrations or longer exposure would incur harm to the photosynthetic system of the leaves.

Key words: index system, green development, Jiangxi Province, regionalization