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厚环乳牛肝菌(Suillus grevillei)N40与绿木霉(Trichoderma virens)T43复合接种下樟子松苗木的生理响应

尹大川1,邓勋2,Ilan Chet3,宋瑞清1**   

  1. (1东北林业大学林学院, 哈尔滨 150040; 2黑龙江省林科院森林保护研究所, 哈尔滨 150040; 3以色列耶路撒冷希伯来大学农学院, 以色列 76100)
  • 出版日期:2014-08-10 发布日期:2014-08-10

Physiological responses of Pinus sylvestris var. mongolica seedlings to the interaction between Suillus grevillei N40 and Trichoderma virens T43.

YIN Da-chuan1, DENG Xu2, Ilan Chet3, SONG Rui-qing1**   

  1. (1School of Forestry, Northeast Forestry University, Harbin 150040, China; 2Forestry Protection Institute of Heilongjiang Forestry Academy, Harbin 150040, China; 3Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel)
  • Online:2014-08-10 Published:2014-08-10

摘要:

采用植物生理学和生物化学等研究方法,研究了厚环乳牛肝菌(Suillus grevillei)N40与绿木霉(Trichoderma virens)T43复合接种条件下,对樟子松苗木的接种效应。测定不同接种方式下樟子松苗木生长指标、菌根合成率、光合色素含量、可溶性蛋白含量、抗氧化酶活性,根际土壤酶活性和抗病保护酶活性等指标。结果表明:N40和T43既有竞争性,同时又具有良好的兼容性,对苗木的生长指标、生理指标均有明显的提高作用。接种N40 30 d后接种T43,3个月后苗木高生长提高43.0%,地径提高55.6%,苗木可溶性蛋白含量提高176.9%,苗木叶绿素a水平提高76.2%,叶绿素b水平提高64.1%,类胡萝卜素水平提高103.6%,苗木CAT和SOD活力分别提高45.5%和43.7%,β-1,3葡聚糖酶和几丁质酶活力提高28.0%和40.0%;苗木根际土壤脲酶和碱性磷酸酶活力分别提高120%和100%。上述结果表明,绿木霉T43能够促进菌根化苗木的生长并能诱导苗木产生抗性。
 

关键词: 大型底栖动物, 异质性, 地统计学, 河流微生境

Abstract: The effects of the interaction between Suillus grevillei and Trichoderma virens on Pinus sylvestris var. mongolica were studied using plant physiology and biochemistry. Seedling growth and physiological parameters were determined, including the biomass, photosynthetic pigment content, soluble protein content, antioxidant enzyme activities, rhizosphere soil enzyme activities and protective enzyme activities. In addition, an optimal resistance system involving T. virens, mycorrhizal fungus (Suillus grevillei) and P. sylvestris var. mongolica seedlings was constructed. Synergies between S. luteus and T. virens were observed and most of the parameters of Pinus sylvestris var. mongolica seedlings inoculated with S. luteus 30 days + T. virens were higher than other treatments. After three months, when compared the control, the S. luteus 30 days + T. virens treatment gave increases in height (43%), collar diameter (55.6%), soluble protein content (176.9%), chlorophyll a (76.2%), chlorophyll b (64.1%), carotenoids (103.6%), CAT activity (45.5%), SOD activity (43.7%), β-1,3glucanase activity (28%), chitinase activity (40%), rhizosphere soil urease activity (120%), and phosphatase activity (100%). These results indicated that there may be a stimulating factor between S. grevillei and T. virens when they are inoculated together (S. grevillei 30 days + T. virens).

Key words: river microhabitat, macroinvertebrate, heterogeneity, geostatistics