This study aimed to determine the effects of Fe limitation on Chlorella vulgaris-Daphnia carinata food chain. C. vulgaris was cultivated with Fe³⁺ at five different concentrations between 0-10000 nmol·L^-1 for 22 days. C. vulgaris cells were collected to feed D. carinata for 14 days. The growth of C. vulgaris and D. carinata was observed. The results showed that the density and chlorophylla content of C. vulgaris were significantly lower at Fe³⁺ concentrations between 0-1000 nmol·L^-1 than those at Fe^3+ concentration of 10000 nmol·L^-1. The decline of Fe³⁺ concentration resulted in that C. vulgaris absorbed less nitrogen but more phosphorus. The body length and the total number of the cumulative eggs of D. carinata decreased gradually with the declining Fe³⁺ concentration. The final body length and total newborns of D. carinata were 3 mm and 93 at 10000 nmol·L^-1 Fe³⁺ concentration, while 2.6 mm and 60 at 0 nmol·L^-1 Fe³⁺ concentration, respectively. It was indicated that Fe limitation had significant effects on the growth of C. vulgaris and its absorption to nitrogen and phosphorus. The growth and reproduction of D. carinata were inhibited under the condition of Fe limitation. These results suggested that the bottomup effects of Fe limitation on C. vulgaris-D. carinata food chain were significant and this would contribute to exploring the effects of Fe on freshwater phytoplankton and zooplankton.