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南方红豆杉对二氧化硫胁迫的生理响应

孙凌霞,张峻铭,蔡仕珍,李西**   

  1. (四川农业大学风景园林学院, 四川成都 611130)
  • 出版日期:2014-07-10 发布日期:2014-07-10

Physiological responses of Taxus chinensis var. mairei to SO2 stress.

SUN Ling-xia, ZHANG Jun-ming, CAI Shi-zhen, LI Xi**   

  1. (College of Landscape Architecture, Sichuan Agricultural University, Chengdu 611130, China)
  • Online:2014-07-10 Published:2014-07-10

摘要: 为探究南方红豆杉(Taxus chinensis var. mairei)对SO2污染的抗逆机理,本实验以1年生盆栽幼苗为材料,采用密闭箱静态熏气法,研究了不同质量浓度SO2(5、10、20和40 mg·m-3)下对南方红豆杉主要逆境生理特性的影响。结果表明,随着SO2浓度的增加和熏气时间的延长,叶片受到不同程度的伤害,同时伴随着叶绿素a和叶绿素b的迅速下降,叶片质膜透性的急剧增加,丙二醛含量大幅度上升,可溶性糖和脯氨酸升高。在同一熏气时间下,随着SO2浓度的增加,SOD、POD、CAT、和APX活性呈现先升高后下降的趋势。在整个熏气时间段内,在5 mg·m-3 SO2胁迫下,SOD和APX活性首先被诱导,而POD和CAT在10 mg·m-3 SO2下被诱导;在40 mg·m-3SO2浓度下,与对照相比,SOD、CAT、APX和POD活性显著下降。可以推断,在低于10 mg·m-3 SO2浓度下,南方红豆杉通过自身的应激保护系统来提高对SO2的抗性,维持正常生长;南方红豆杉适用于SO2污染区的园林绿化。

关键词: 土壤微生物量碳氮, 高寒草甸, 酶, 退化, 碳源代谢

Abstract: In order to investigate the resistance mechanism of Taxus chinensis var. mairei to sulfur dioxide (SO2) pollution, one-year-old potted seedlings were grown in an enclosed fumigation chamber, and the concentration of SO2 was set as 5, 10, 20 and 40 mg·m-3 for various durations. Our results showed that the membrane permeability, the contents of  malondialdehyde (MDA), freeproline and soluble sugar were enhanced with the increase of SO2 concentration and duration, while the contents of chlorophyll a and b decreased. The injury symptoms in leaves were first observed at 20 mg·m-3 SO2 after 5-day exposure. Under the same experimental duration, the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) were induced greatly at the lower SO2 concentrations and then decreased at the higher SO2 concentrations. The activities of SOD and APX were induced at 5 mg·m-3 SO2, while those of peroxidase (POD) and catalase (CAT) were induced at 10 mg·m-3 SO2. Under the 40 mg·m-3 SO2 stress, the activities of SOD, CAT, POD, and APX were significantly decreased in comparison with those in the control during the whole experimental periods. Our results suggest that Taxus chinensis var. mairei can adapt to 10 mg·m-3 SO2polluted environments with no obvious phenotypic injury through its own stress protection system to improve resistance to SO2, therefore this species can be planted in SO2 polluted areas.

Key words: carbon metabolic index, degradation, soil microbial biomass C and N, alpine meadow, enzymes