欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志

• 研究报告 • 上一篇    下一篇

一株拮抗立枯丝核菌的放线菌筛选、鉴定及生理特性

张靖宜1,2,张倩茹2**,项文化1,魏树和2,牟文燕2   

  1. (1中南林业科技大学生命科学与技术学院, 长沙 410004; 2中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室, 沈阳 110016)
  • 出版日期:2014-02-10 发布日期:2014-02-10

Screening, identification and physiological characteristics of an actinomycete strain against Rhizoctonia solani.

ZHANG Jing-yi1,2, ZHANG Qian-ru2**, XIANG Wen-hua1, WEI Shu-he2, MU Wen-yan2   

  1. (1Faculty of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China; 2 Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China)
  • Online:2014-02-10 Published:2014-02-10

摘要:

通过对峙实验,从采集自黄渤海海域的双齿围沙蚕(Perinereis aibuhitensis)中分离到一株内生放线菌SCF-18,并对其抑菌活性进行分析。SCF-18对立枯丝核菌(Rhizoctonia solani)和小麦根腐病菌(Bipolaris sorokiniana)都具有拮抗作用,且对立枯丝核菌生长的拮抗效果最为明显,生长抑制率为89.78%。抑菌试验结果表明:菌株SCF-18发酵滤液可以抑制立枯丝核菌丝的生长,发酵液浓度越高,抑制力越强;当发酵液浓度达到50%时,对两种病原菌的抑制率分别为89.78%和80.26%。菌株SCF-18生长特性试验结果表明:菌株SCF-18生长最适温度为30 ℃,最适pH值为7;放线菌SCF-18耐盐度可达5%。根据形态特征、培养特征、生理生化特性和16S rDNA序列分析,将放线菌SCF-18初步鉴定为淡紫灰链霉菌(Streptomyces lavendulae)。本研究发现,SCF-18菌株是防治立枯丝核菌等病原菌的潜在优良生防菌株,具有潜在的开发应用价值。
 

 

关键词: 种子活力, 种子发育, 小麦, 环境温度

Abstract: By using dual culture technique, a strain of endophytic actinomycete named SCF-18 was screened out and isolated from Perinereis aibuhitensis collected from the Huanghai and Bohai Sea, and the bioactivities of the actinomycete against pathogenic fungi were determined. The strain SCF18 showed significantly antagonistic activities to both Rhizoctonia solani and Bipolaris sorokiniana, particularly more potent to R. solani with the maximum inhibition rate of 89.78%. The fermentation filtrate of SCF-18 could inhibit the growth of R. solani in a dosedepended manner. Furthermore, the fermentation filtrate of SCF-18 in 50% concentration showed the maximum growth inhibitory effects to R. solani and H. sativum with inhibition rates of 89.78% and 80.26%, respectively. The optimum growth condition for SCF-18 was at 30 ℃ with pH 7. The highest tolerance of salinity of SCF-18 was 5%. Based on the analysis of morphology, cultural characteristics, physiological and iochemical properties, and 16S rDNA sequencing, the SCF-18 was identified as Streptomyces lavendula. Our results suggest that the strain SCF-18 could be valuable in the biocontrol of R. solani.

Key words: environmental temperature, seed vigor, wheat., seed development