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原绿球藻固定化培养去除NH4+-N的效果

毛欣欣,蒋霞敏**,钱鹏   

  1. (宁波大学海洋学院, 浙江宁波 315211)
  • 出版日期:2014-11-10 发布日期:2014-11-10

Effect of immobilized Prochlorococcus culture on NH4+-N removal.

MAO Xin-xin, JIANG Xia-min**, QIAN Peng   

  1. (School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China)
  • Online:2014-11-10 Published:2014-11-10

摘要:

为了探究固定化微藻去除污水中NH4+-N的效果,以原绿球藻(Prochlorococcus)为藻种,采用褐藻胶包埋技术,进行了不同藻球密度(0、100×104、300×104、500×104、700×104、900×104 cells·ball-1)、不同藻球用量(固定化藻球与人工污水的体积比V/V藻球为4∶1、3∶1、2∶1、1∶1)及藻球加固工艺(CaCl2加固)等对比试验。结果表明:固定化藻比悬浮藻生长缓慢,去除NH4+-N的速率小于悬浮藻;固定化藻球藻细胞密度越高,去除NH4+-N的效果越好,但考虑单位藻细胞去除率,包埋密度以700×104 cells·ball-1为宜;经N饥饿处理的藻细胞去除NH4+-N效果显著优于未饥饿组;固定化藻球用量大,去除NH4+-N越快,V/V藻球为1∶1培养3 d后去除率可达100%;固定化藻球定期加固,有利于延长其使用寿命,增加对NH4+ -N的吸收量。
 

关键词: 多样性, 贡嘎山, 甲烷氧化菌群落, 植被类型

Abstract: Immobilization of microalgae Prochlorococcus with sodium alginate embedding was carried to explore the removal effect of NH4+-N from artificial sewage water. Different algae ball densities (0, 100×104, 300×104, 500×104, 700×104, 900×104 cells·ball-1), different dosages of algae balls (the ratio of algae balls and sewage 4∶1, 3∶1, 2∶1, 1∶1) and algae ball reinforcement with CaCl2 were applied in the experiment. The results showed that the growth rate of the immobilized algae and its removal ratio of NH4+-N were lower than those of suspended ones. The NH4+-N removal effects of the immobilized algae were dosedependent; however, considering the average removal ratio of algal cell, 700×104 cells·ball-1 was the best cell density. The algae cells with N-starvation treatment showed much better removal effect than the untreated cells. The more the immobilized algae balls were used, the quicker removal of NH4+-N was. After three days’ cultivation, the removal effect reached up to 100% with the volume ratio of the immobilized algae balls and artificial sewage of 1∶1. With CaCl2 reinforcement, the algae balls worked longer and assimilated much more NH4+-N.

Key words: Gongga Mountain, methanotrophic bacterial community, vegetation, diversity