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土地利用方式对土壤细菌、泉古菌和氨氧化古菌丰度的影响

王影1,2,张志明2,李晓慧2,严君2,韩晓增1,2**   

  1. (1东北农业大学资源环境学院, 哈尔滨 150030; 2中国科学院东北地理与农业生态研究所黑土区农业生态重点实验室, 哈尔滨 150081)
  • 出版日期:2013-11-10 发布日期:2013-11-10

Effects of land use type on the abundance of bacteria, crenarchaea, and ammonia-oxidizing archaea in black soil. 

WANG Ying1,2, ZHANG Zhi-ming2, LI Xiao-hui2, YAN Jun2, HAN Xiao-zeng1,2**   

  1. (1College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China; 2 Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China)
  • Online:2013-11-10 Published:2013-11-10

摘要:

为评价微生物主要类群在土壤生态系统中的作用,本文以长期定位试验为研究平台,选取草地、农田(大豆、玉米和小麦)和裸地试验5个处理,采用实时荧光定量PCR技术,研究了黑土中细菌、泉古菌和氨氧化古菌(AOA)的丰度变化。结果表明:草地细菌、泉古菌和AOA的丰度显著高于农田和裸地。而农田土壤不同作物对细菌、泉古菌和AOA丰度影响不显著。草地土壤的细菌丰度比裸地、大豆、玉米和小麦分别增加了62.7%、29.0%、41.1%和18.8%,泉古菌的丰度分别增加了34.4%、30.7%、37.6%和33.7%,AOA的丰度增加了28.6%、42.6%、44.4%和53.6%。土壤细菌丰度与土壤pH值和有机碳含量均呈正相关(r=0.935, P<0.01;r=0.887,P<0.05);泉古菌的丰度与土壤pH值亦显著正相关(r=0.845, P<0.05);而土壤AOA的丰度与土壤pH值和有机碳含量相关性不显著。
 

关键词: 气候因子, 遥感, 地统计, 空间格局, 森林凋落量

Abstract: Taking a long-term experiment in the black soil area of Northeast China as the platform, this paper studied the abundance of soil bacteria, crenarchaea, and ammoniaoxidizng archaea in grassland, cropland (soybean, corn, and wheat), and bare land by targeting the 16S rRNA gene and amoA gene with real-time PCR approaches, aimed to evaluate the functions of main microbial groups in soil ecosystem under different land use types. The results showed that the abundance of soil bacteria, crenarchaea, and ammonia-oxidizing archaea was significantly higher in grassland than in cropland and bare land, but had no significant difference in different crop lands. As compared with that in bare land, soybean land, corn land, and wheat land, the abundance of bacteria, crenarchaea, and ammonia-oxidizing archaea in grassland was increased by 62.7%, 29.0%, 41.1% and 18.8%, 34.4%, 30.7%, 37.6% and 33.7%, and 28.6%, 42.6%, 44.4% and 53.6%, respectively. The abundance of soil bacteria had significant positive correlations with soil pH (r=0.935, P<0.01) and organic carbon content (r=0.887, P<0.05), that of soil crenarchaea was significantly positively correlated with soil pH (r=0.845,P<0.05), whereas the abundance of soil ammonia-oxidizing archaea had no significant correlations with soil pH and organic carbon content.

Key words: forest litterfall, geostatistics, climatic factor, model, spatial pattern, remote sensing.