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生态学杂志 ›› 2012, Vol. 31 ›› Issue (5): 1104-1110.

• 研究报告 • 上一篇    下一篇

五种淡水微藻的适宜培养温度和光照强度

严佳琦,黄旭雄**,陶妍,温文,刘林林,曾蓓蓓   

  1. (上海海洋大学水产与生命学院, 上海 201306)
  • 出版日期:2012-05-10 发布日期:2012-05-10

Suitable temperature and light intensity for culturing five species of freshwater microalgae.

YAN Jia-qi, HUANG Xu-xiong, TAO Yan, WEN Wen, LIU Lin-lin, ZENG Bei-bei   

  1. (College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China)
  • Online:2012-05-10 Published:2012-05-10

摘要: 从淡水中分离得到绿球藻(Chlorococcumsp.)SHOU-F3、纤维藻(Ankistrodesmus sp.)SHOU-F33、小球藻(Chlorella sp.)SHOU-F46、空星藻(Coelastrum sphaericum)SHOU-F10和栅藻(Scenedesmus sp.)SHOU-FX,分别在光照培养箱中研究了温度、光照强度对5种微藻增殖的影响,并分析了5种微藻的细胞组成。结果表明:绿球藻SHOU-F3、纤维藻SHOU-F33、小球藻SHOU-F46、空星藻SHOU-F10和栅藻SHOU-FX的最适生长温度分别为29.8、23.5、31.4、34.4和24.7 ℃;最适光照强度分别为16、119、42、82和106 umol·m-2·s-1;在适宜培养条件下,绿球藻SHOU-F3的色素、蛋白以及总糖的百分含量最高,纤维藻SHOU-F33的脂肪百分含量最高。

关键词: 温带草原, 细菌多样性, 细菌群落结构, 氮沉降, 降水

Abstract: In this study, five species of microalgae (Chlorococcumsp.SHOU-F3, Ankistrodesmus sp. SHOU-F33, Chlorellasp.SHOU-F46, Coelastrum sphaericumSHOU-F10, and Scenedesmus sp. SHOU-FX) were isolated from freshwater, and a culture experiment was conducted to determine the optimal water temperature and light intensity for the five microalgae. The biochemical compositions of these microalgae were also analyzed. For the Chlorococcumsp. SHOU-F3,Ankistrodesmus sp. SHOU-F33, Chlorellasp. SHOU-F46, C. sphaericum SHOU-F10, and Scenedesmus sp. SHOU-FX, the optimal water temperature was 29.8, 23.5, 31.4, 34.4, and 24.7 ℃, and the optimal light intensity was 16, 119, 42, 82, and 106 umol·m-2·s-1, respectively. Under the suitable culture conditions, Chlorococcum sp. SHOU-F3 cells had the highest contents of pigments, protein, and carbohydrates, while Ankistrodesmus sp. SHOU-F33 cells had the highest content of total lipid.

Key words: temperate grassland, bacterial diversity, bacterial community structure, nitrogen deposition, precipitation.