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生态学杂志 ›› 2012, Vol. 31 ›› Issue (08): 1911-1916.

• 研究报告 • 上一篇    下一篇

孑遗植物水松不同年龄级种群遗传多样性的ISSR分析

吴则焰1,刘金福2**,洪伟2,潘东明3,郑世群2,何中声2   

  1. 1福建农林大学生命科学学院, 福州 350002; 2福建农林大学林学院, 福州 350002; 3福建农林大学园艺学院, 福州 350002)
  • 出版日期:2012-08-10 发布日期:2012-08-10

Genetic diversity of different lifestage population of Glyptostrobus pensilis, an endangered plant in China: ISSR analysis.

WU Ze-yan1,2, LIU Jin-fu2**, HONG Wei2, PAN Dong-ming3, ZHENG Shi-qun2, HE Zhong-sheny2   

  1. (1College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2College of Forest, Fuzhou 350002, China; 3College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
  • Online:2012-08-10 Published:2012-08-10

摘要: 按胸径将福建省屏南水松(Glyptostrobus pensilis)种群划分为成树、小树、幼苗3个年龄级,利用ISSR分子标记对不同年龄级的水松遗传多样性及遗传结构进行分析,旨在揭示其不同世代间遗传多样性的变化规律,为水松资源有效保护提供科学依据。用10条随机引物共检测到83个扩增位点,其中多态位点32个,多态位点百分率(P)为38.55%。同其他濒危裸子植物相比,水松具有较低的遗传多样性。不同年龄级的遗传多样性差别较大,P、Nei基因多样度(He)、Shannon信息指数(I)均以成树最高,小树次之,幼苗最低,表明水松种群遗传多样性世代间呈现衰退趋势。分子方差分析(AMOVA)表明,水松种群不同年龄级内、年龄级间均存在遗传变异,但遗传变异主要存在于年龄级内。不同年龄级间的遗传分化系数(Gst)为0.2872,基因流(Nm)仅0.6204,遗传相似度以幼苗和小树最高。基于水松种群遗传学和生态学的研究结果,提出应加大对遗传多样性高的水松种群保护力度,加强水松种群基因交流,以最大限度地保存水松资源的遗传多样性。

关键词: 干旱, 水稻, 湿润指数距平率, 分布特征, 西南地区

Abstract: Glyptostrobus pensilis is a rare and endangered species in China, but few studies have been made on the conservation genetics of this species. In this study, the G. pensilis population in Pingnan of Fujian Province, East China was divided into 3 life stages, i.e., adult, juvenile, and seedling, according to the size class, and the genetic diversity and genetic differentiation of different life stage G. pensilis were investigated by using intersimple sequence repeat (ISSR) markers. Ten ISSR primers were used to amplify 72 individuals in the population, and 83 discernible DNA fragments were produced, of which, 32 were polymorphic loci (the percentage of polymorphic loci (P) = 38.55%), suggesting the low level genetic diversity of the population. The difference of the genetic diversity among the life stages was evident. Adult had the highest P, Nei’s gene index (He), and Shannon information index (I), followed by juvenile, and seedling, suggesting that the genetic diversity among the life stages presented a declining trend. The analysis of molecular variance (AMOVA) showed that the genetic diversity might exist within and among the life stages, mainly within the life stages. Moreover, the genetic differential index (Gst) among the life stages was 0.2872, while the gene flow (Nm) was 0.6203. The genetic identity between seedlings and juveniles was the highest. Based on the analysis of population genetic structure and the community investigation, it would be necessary to establish a conservation site in the optimal habitat of G. pensilis and to crosstransplant adult plants and seedlings mutually to enhance the gene flow. By this means, the genetic diversity resources of the species could be preserved to the greatest extent.

Key words: drought, rice, wetness index anomaly rate, distribution characteristic, Southwest China.