邻苯二甲酸二乙基己酯对翡翠贻贝(Perna viridis)生化指标的影响

生态学杂志 ›› 2012, Vol. 31 ›› Issue (01): 100-105.

邻苯二甲酸二乙基己酯对翡翠贻贝(Perna viridis)生化指标的影响

蔡文贵1,2,秦洁芳2,3,贾晓平2,陈海刚2,陈炜婷4,马胜伟2,张喆2,倪朝辉1**

1 中国水产科学研究院淡水生态与健康养殖重点开放实验室，中国水产科学研究院长江水产研究所，武汉 430223；2农业部南海渔业资源环境重点野外科学观测实验站，广东省渔业生态环境重点研究室，中国水产研究院南海水产研究所，广州 510300；3广东省农业科学院土壤肥料研究所，广州 510640；4广州大学环境科学与工程学院，广州 510006

出版日期:2012-01-08 发布日期:2012-01-08

Effects of di-(2-ethylhexyl) phthalate on the biochemical indices of green mussel (Perna viridis).

CAI Wen-gui1,2, QIN Jie-fang2,3, JIA Xiao-ping2, CHEN Hai-gang2, CHEN Wei-ting4, MA Sheng-wei2, ZHANG Zhe2, NI Chao-hui1**

1 The Key Laboratory of Freshwater Ecology and Healthy Aquaculture, CAFS, Wuhan 430223, China;2 Key Scientific Observation Station for South China Sea Fishery Resources and Environment, Ministry of Agriculture; Key Laboratory of Fishery Ecology and Environment, Guangdong Province of China; South China Sea Fisheries Research Institute, CAFS; Guangzhou 510300, China;3 Soil and Fertilizer Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;4 School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China

Online:2012-01-08 Published:2012-01-08

摘要/Abstract

摘要： 实验条件下，研究邻苯二甲酸二乙基己酯（DEHP）5个浓度组（0、0.38、1.92、9.60和48.00 mg·L^-1）长时间胁迫下翡翠贻贝（Perna viridis）内脏团和外套膜中抗氧化酶（SOD和CAT）活性和丙二醛（MDA）含量的变化，以及胁迫解除后这些指标的恢复情况。结果表明：在胁迫过程中，翡翠贻贝内脏团SOD活性表现为先显著升高，随后受抑制而逐渐降低（P<0.05），CAT活性则表现为先被抑制后受诱导，15 d后恢复到对照组水平，MDA含量呈显著增加的趋势（P<0.05）；外套膜中的SOD活性在胁迫初期在低浓度组被抑制，而在高浓度组则被诱导（P<0.05），4 d后SOD活性逐渐恢复到对照组水平，各浓度组MDA含量均出现明显的增加（P<0.05）；净化阶段，低浓度组（0.38 mg·L^-1）内脏团SOD活性和CAT活性逐渐恢复到对照组水平，但MDA含量升高；净化7 d后，除高浓度组（48.00 mg·L^-1）外，其余浓度组外套膜中SOD活性均已经恢复到对照组水平，MDA含量也没有出现明显升高的现象。研究表明，DEHP对翡翠贻贝内脏团和外套膜抗氧化防御系统酶具有明显的影响，DEHP诱导引起2种组织内脂质过氧化损伤，并且短期内这种损伤无法消除。

关键词: 径向差异, 热扩散技术, 大气蒸发潜力, 日变化格局

Abstract: A laboratory experiment was carried out to study the variations of superoxide dismutase (SOD) and catalase (CAT) activities and malondialdehyde (MDA) content in the visceral mass and mantle of green mussel (Perna viridis) exposed to different concentrations (0, 0.38, 1.92, 9.60, and 48.00 mg·L^-1) of di-(2-ethylhexyl) phthalate (DEHP) for a long term, and the recovery of these indices after the stress released. During the exposure period, the SOD activity in the visceral mass had an initial significant increase (P<0.05) and followed by a gradual decrease, while the CAT activity was inhibited first but induced then, recovering to the level of the control after 15 days of exposure. The MDA content in the visceral mass increased significantly with increasing exposure time (P<0.05). In the early period of exposure, the SOD activity in the mantle of the P. viridis groups exposed to lower concentrations DEHP was inhibited but that of the groups exposed to higher concentrations DEHP was induced (P<0.05), while after 4 days of exposure, the SOD activity of all the groups recovered to the normal level. The MDA content in the mantle of all the groups increased significantly (P<0.05). During the releasing period, the SOD and CAT activities in the visceral mass of P. viridis exposed to 0.38 mg·L^-1 of DEHP recovered gradually to the normal level, but the MDA content increased. After 7 days of releasing, the SOD activity in the mantle of P. viridis exposed to all test concentrations (except 48.00 mg·L^-1) of DEHP recovered to the normal level, while the MDA content maintained at a stable level. The above results indicated that DEHP had no obvious effects on the enzymes of antioxidant defense systems in the visceral mass and mantle of P. viridis but could induce the lipid peroxidation damage in the 2 tissues, and this damage could not recover in a short term.

Key words: radial difference, thermal dissipation probe, atmospheric potential evapotranspiration, diurnal pattern.

蔡文贵,秦洁芳,贾晓平,陈海刚,陈炜婷,马胜伟,张喆,倪朝辉. 邻苯二甲酸二乙基己酯对翡翠贻贝(Perna viridis)生化指标的影响[J]. 生态学杂志, 2012, 31(01): 100-105.