• 研究报告 •

### 邻苯二甲酸二乙基己酯对翡翠贻贝(Perna viridis)生化指标的影响

1. 1 中国水产科学研究院淡水生态与健康养殖重点开放实验室， 中国水产科学研究院长江水产研究所， 武汉 430223；2农业部南海渔业资源环境重点野外科学观测实验站， 广东省渔业生态环境重点研究室， 中国水产研究院南海水产研究所， 广州 510300；3广东省农业科学院土壤肥料研究所， 广州 510640；4广州大学环境科学与工程学院， 广州 510006
• 出版日期:2012-01-08 发布日期:2012-01-08

### Effects of di-(2-ethylhexyl) phthalate on the biochemical indices of green mussel (Perna viridis).

CAI Wen-gui1,2, QIN Jie-fang2,3, JIA Xiao-ping2, CHEN Hai-gang2, CHEN Wei-ting4, MA Sheng-wei2, ZHANG Zhe2, NI Chao-hui1**

1. 1 The Key Laboratory of Freshwater Ecology and Healthy Aquaculture, CAFS, Wuhan 430223, China;2 Key Scientific Observation Station for South China Sea Fishery Resources and Environment, Ministry of Agriculture; Key Laboratory of Fishery Ecology and Environment, Guangdong Province of China; South China Sea Fisheries Research Institute, CAFS; Guangzhou 510300, China;3 Soil and Fertilizer Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;4 School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China
• Online:2012-01-08 Published:2012-01-08

Abstract: A laboratory experiment was carried out to study the variations of superoxide dismutase (SOD) and catalase (CAT) activities and malondialdehyde (MDA) content in the visceral mass and mantle of green mussel (Perna viridis) exposed to different concentrations (0, 0.38, 1.92, 9.60, and 48.00 mg·L-1) of di-(2-ethylhexyl) phthalate (DEHP) for a long term, and the recovery of these indices after the stress released. During the exposure period, the SOD activity in the visceral mass had an initial significant increase (P<0.05) and followed by a gradual decrease, while the CAT activity was inhibited first but induced then, recovering to the level of the control after 15 days of exposure. The MDA content in the visceral mass increased significantly with increasing exposure time (P<0.05). In the early period of exposure, the SOD activity in the mantle of the P. viridis groups exposed to lower concentrations DEHP was inhibited but that of the groups exposed to higher concentrations DEHP was induced (P<0.05), while after 4 days of exposure, the SOD activity of all the groups recovered to the normal level. The MDA content in the mantle of all the groups increased significantly (P<0.05). During the releasing period, the SOD and CAT activities in the visceral mass of P. viridis exposed to 0.38 mg·L-1 of DEHP recovered gradually to the normal level, but the MDA content increased. After 7 days of releasing, the SOD activity in the mantle of P. viridis exposed to all test concentrations (except 48.00 mg·L-1) of DEHP recovered to the normal level, while the MDA content maintained at a stable level. The above results indicated that DEHP had no obvious effects on the enzymes of antioxidant defense systems in the visceral mass and mantle of P. viridis but could induce the lipid peroxidation damage in the 2 tissues, and this damage could not recover in a short term.