关键词: 大豆, 生长, 紫外线-B(UV-B), 镉(Cd), 抗氧化酶, 吲哚乙酸(IAA), 氧化酶

Abstract: To quickly detect bacterial physiological characters, the authors developed a kit to test the utilization of various carbon sources by bacteria and their responses to medium pH and antibiotics. In the kit, 95 kinds of carbon sources were utilized for target bacteria, and the indicator MTT was used to test the utilization of specific carbon sources by the target bacteria. The growth of target bacteria at a series of medium initial pH and NaCl concentration and the sensitivities of the bacteria to various kinds and different concentrations of antibiotics were also tested. For result observation, an optimization was made on the MTT concentration, medium solidification, bacterial inoculation amount, and observation duration. Accordingly, a kit with BiobiqA (C-source utilizing spectra) and BiobiqB (antimicrobial sensitivity pattern) was developed. The test and validation with model bacteria (Enterobacter cloacae ATCC13047^T and Rhizobium NZP2213^T) showed that our newly developed kit had the advantages of broad spectrum, easy operation, high accuracy, cost-saving, and time-saving, being able to rapidly detect the physiological characters of bacteria

Key words: Soybean, Ultraviolet-B(UV-B), Cadmium(Cd), SOD, POD, Indole-3-acetic acid(IAA)oxidase