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生态学杂志 ›› 2010, Vol. 29 ›› Issue (07): 1340-1346.

• 研究报告 • 上一篇    下一篇

柠檬酸和草酸对Pb胁迫下马蔺生长和生理的影响

王鸿燕,佟海英**,黄苏珍,原海燕   

  1. 江苏省中国科学院植物研究所,南京中山植物园, 南京 210014
  • 出版日期:2010-07-08 发布日期:2010-07-08

Effects of citric acid and oxalic acid on the growth and physiology of Iris lactea var. chinensis  under Pb stress.

WANG Hong-yan, TONG Hai-ying, HUANG Su-zhen, YUAN Hai-yan   

  1. Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing Botanical Garden, Nanjing 210014,China
  • Online:2010-07-08 Published:2010-07-08

摘要: 采用营养液培养方法,研究了外源柠檬酸(0.5、5 mmol·L-1)和草酸(0.5、5 mmol·L-1)对不同浓度Pb(0、100、500 mg·L-1)胁迫下马蔺(Iris lactea var. chinensis)生长和生理的影响。结果表明,与单独Pb胁迫相比,加入5 mmol·L-1的柠檬酸显著促进了马蔺株高的生长;草酸的加入缓解了500 mg·L-1 Pb胁迫对马蔺根生长的影响;加入0.5 mmol·L-1的草酸缓解了500 mg·L-1 Pb胁迫对马蔺地上部生长的影响,并显著增加了100和500 mg·L-1 Pb胁迫下马蔺的地下部干质量;0.5 mmol·L-1的柠檬酸能显著增加100 mg·L-1 Pb胁迫下马蔺叶片SOD酶活性;0.5和5 mmol·L-1柠檬酸和0.5 mmol·L-1草酸均能显著增加100 mg·L-1 Pb胁迫下马蔺体内POD酶活性;5 mmol·L-1的柠檬酸和0.5 mmol·L-1的草酸均缓解了500 mg·L-1 Pb胁迫对马蔺光合色素的影响;0.5和5 mmol·L-1的柠檬酸和0.5 mmol·L-1的草酸均能缓解由100和500 mg·L-1Pb胁迫引起马蔺叶片细胞膜的损伤。

关键词: 城市森林, 关联性, 生长指数, 组合

Abstract: A hydroponic experiment was conducted to study the effects of exogenous citric acid (0.5 and 5 mmol·L-1) and oxalic acid (0.5 and 5 mmol·L-1) on the growth and physiology of Iris lactea var. chinensis  under 100 and 500 mg·L-1 Pb stress. Comparing with Pb stress alone, adding 5 mmol·L-1of citric acid promoted the shoot growth of I. lactea var. chinensis significantly. The addition of oxalic acid mitigated the impact of 500 mg·L-1Pb stress on the root growth of I. lactea  var. chinensis. Adding 0.5 mmol·L-1of oxalic acid alleviated the 500 mg·L-1Pb stress on the shoot growth, and increased the root dry mass significantly under 100 and 500 mg·L-1 Pb stress. The SOD activity in I. lactea var. chinensis  leaves under 100 mg·L-1Pb stress was increased significantly by adding 0.5 mmol·L-1of citric acid, and the POD activity of  I. lactea var. chinensis under 100 mg·L-1 Pb stress was increased significantly by adding 0.5 and 5 mmol·L-1 of citric acid or 0.5 mmol·L-1of oxalic acid. The impact of 500 mg·L-1 Pb stress on the photosynthetic pigments of I. lactea var. chinensis was alleviated by the addition of 5mmol·L-1 of citric acid or 0.5 mmol·L-1of oxalic acid. Adding 0.5 and 5 mmol·L-1of citric acid or 0.5 mmol·L-1 of oxalic acid reduced the membrane damage of I. lactea var. chinensis leaves under 100 and 500 mg·L-1 Pb stress.

Key words: Urban forest, Correlation, Growth index, Compounding