关键词: 桃蚜, 吡虫啉, 次亚致死剂量弥雾, 生物测定, 时间-剂量-死亡率模拟

Abstract: The eastern Wandashan Mountains of Heilongjiang Province is one of theareas with high population density of wapiti (Cervus elaphus xanthopygus).
In this paper, 167 wapiti fecal samples were collected there in two winters to identify their DNA based on 7 microsatellite loci, aimed to study the genetic div
ersity of the population, and the effects of the population’s dramatic decline in recent years on the population genetic structure. The DNA of the samples was
assigned to 66 individuals. The average numbers of the alleles and effective alleles were 9.00±2.77 and 3.97±0.99, respectively, the average polymorphism information content was 0.69±0.09, and the average observed and expected heterozygosity values were 0.69±0.08 and 0.74±0.08, respectively. The population was significantly deviated from Hardy-Weinberg Equilibrium, but the inbreeding coefficient was 0.060, not significantly deviated from zero. It was suggested that the genetic diversity of the population was still higher, and the dramatic decline of the population didn’t yet affect the population genetic structure due to time lag.

Key words: Myzus persicae, Imidacloprid, Low sublethal sprays, Bioassay, Time-concentration-mortality modeling