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生态学杂志 ›› 2009, Vol. 28 ›› Issue (10): 2002-2008.

• 研究报告 • 上一篇    下一篇

五节芒定居对尾矿砂微生物群落结构的影响

陈友静;陈家元;杨静丹;张崇邦**   

  1. 台州学院生命科学学院,浙江临海 317000
  • 出版日期:2009-11-10 发布日期:2009-11-10


Effects of Miscanthus floridulus inhabitation on microbial community structure in mine tailings.

CHEN You-jing;CHEN Jia-yuan;YANG Jing-dan;ZHANG Chong-bang   

  1. School of Life Sciences, Taizhou College, Linhai 317000, Zhejiang, China
  • Online:2009-11-10 Published:2009-11-10

摘要: 根据五节芒(Miscanthus floridulus)定居盖度(0、17%、41%、71%和89%),选择了5个尾矿砂研究样地,并分析了尾矿砂微生物群落结构动态及其与土壤理化特性、重金属含量和植物生物量的关系。结果表明:随着五节芒在尾矿砂上的定居,除pH以外,尾矿砂的有机碳、总氮、总磷、NH4+-N、NO3--N、速效磷的含量、团聚体稳定性和最大持水量均显著性提高,而土壤重金属总量与DTPA可提取量均显著性下降。主分量分析表明,随着五节芒定居,尾矿砂微生物群落总体结构发生了显著变化,其中革兰氏阳性细菌、革兰氏阴性细菌、真菌、放线菌、菌根菌(16∶1ω5c和20∶3ω3)、藻类脂肪酸含量以及微生物群落多样性(香农维纳指数)均显著提高,并且与一些土壤理化特性,如NO3--N、速效磷、团聚体稳定性、土壤全氮和五节芒地上、地下生物量存在着显著或极显著正相关关系。仅有革兰氏阴性细菌和菌根菌脂肪酸含量分别与DTPA铜呈显著负相关。

关键词: 冬小麦, 小量播种, 产量效应, 分蘖, 生理功能, 代谢活性, 株穗数

Abstract: Five sampling sites of mine tailings inhabited by Miscanthus floridulus with the coverage of 0, 17%, 41%, 71%, and 89% were selected to analyze the ynamics of microbial community structure and its relationships with mine tailings’ physical and chemical properties, heavy metals contents, and plant biomass. With the inhabitation of M. floridulus, the mine tailings’ organic carbon, total nitrogen, total phosphorus, NH4+-N, NO3--N, and available phosphorus contents, ggregate stability, and maximum waterholding capacity increased significantly, but the total and DTPAextractable heavy metals contents  significantly decreased. The principal component analysis indicated that the microbial community structure changed significantly with the inhabitation of M. floridulus. The Grampositive bacterial fatty acids, Gramnegative bacterial fatty acids, fungal fatty acid, actinomycete fatty acid, mycorrhizal fatty acids (16∶1ω5c and 20∶3ω3), algal fatty acids, and microbial community diversity (ShannonWiener index) all increased significantly and had significant positive correlations with the mine tailings’ total nitrogen, NO3--N and available phosphorus contents, aggregate stability, and the aboveground and belowground biomass of M. floridulus. Only the Gramnegative bacterial and mycorrizal phospholipid fatty acids were negatively correlated with mine tailings’ DTPAextractable Cu content.

Key words: Winter wheat, Small amount precision seeding, Yield, Tiller, Physiological function, Metabolism activity, Spike per plant