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生态学杂志 ›› 2009, Vol. 28 ›› Issue (09): 1794-1800.

• 研究报告 • 上一篇    下一篇

盐胁迫对二色补血草种子萌发的影响

刘玉艳1**;王辉1;于凤鸣2;李淑洁1   

  1. 1河北科技师范学院园艺园林系|河北昌黎 066600;2河北科技师范学院生命科学系|河北昌黎 066600
  • 出版日期:2009-09-10 发布日期:2009-09-10

Effects of salt stress on Limonium bicolor seed germination.

LIU Yu-yan1;WANG Hui1;YU Feng-ming2|LI Shu-jie1   

  1. 1Department of Horticulture and Gardens, Hebei Normal University of Science and Technology, Changli 066600, Hebei, China|2Department of Life Science, Hebei Normal University of Science and Technology, Changli 066600, Hebei, China
  • Online:2009-09-10 Published:2009-09-10

摘要: 以二色补血草(Limonium bicolor)种子为试验材料,用不同浓度的NaCl、Na2SO4及二者质量比为1:1的混合盐在其种子萌发过程中进行胁迫,调查盐胁迫对种子萌发的影响,并测定萌发过程中种子蛋白质含量、淀粉酶活力及还原糖和总糖含量的变化。结果表明:盐胁迫使二色补血草种子的发芽势、发芽指数和发芽率明显降低,而且随着盐浓度的增加,抑制作用增强;3种盐对种子萌发的抑制作用强度依次为NaCl>混合盐>Na2SO4;二色补血草种子耐盐适宜范围为Na2SO4 1.2%、混合盐0.8%、NaCl 0.5%,耐盐半致死浓度为Na2SO42.1%、混合盐1.4%、NaCl 1.1%,耐盐极限浓度为Na2SO43.5%、混合盐2.4%、NaCl 2.0%。盐处理后不萌发的种子转入蒸馏水后重新萌发的试验结果表明,盐分胁迫主要是通过渗透效应,抑制种子中蛋白质和总糖的分解,进而延缓或抑制了种子的萌发。

关键词: 绞股蓝, 蒸腾速率, 气孔导度, 光量子通量密度, 净光合速率

Abstract: This paper studied the effects of different concentration NaCl, Na2SO4, and 1:1 NaCl+Na2SO4 on the seed germination of Limonium bicolor, and the variations in the seed protein content, amylase activity, and reducing sugar and total soluble sugar contents during the process of seed germination. Under the stress of test salts, the germination potential, germination index and germination rate of L. bicolor seeds decreased obviously, and the inhibition effect was increased with increasing salt concentration. The inhibition effect was in the sequence NaCl>NaCl+Na2SO4>Na2SO4. The fit salt concentration for L. bicolor seed germination was 1.2% Na2SO4, 0.8% NaCl+Na2SO4, and 0.5% NaCl, and the half-lethal concentration and fatal concentration of Na2SO4, NaCl+Na2SO4, and NaCl was 2.1% and 3.5%, 1.4% and 2.4%, and 1.1% and 2.0%, respectively. The regermination test of transferring nongerminated seeds after salttreatment into distilled water showed that salt stress mainly inhibited the decomposition of seed protein and sugar via osmosis effect, and further, delayed or inhibited the seed germination.

Key words: Gynostemma pentaphyllum, Transpiration rate, Stoma conductance, Photon flux density, Net photosynthetic rate